Guo Suqin, Zhang Wei, He Lishan, Tan Guiyu, Min Myo, Kyaw Myat Phone, Wang Baomin, Cui Liwang
College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.
Myanmar Medical Association, No. 249, Thein Phyu Road, Mingalartaungnyunt Township, Yangon, Myanmar.
Anal Bioanal Chem. 2016 Sep;408(22):6003-8. doi: 10.1007/s00216-016-9363-9. Epub 2016 Feb 12.
Artesunate is a frontline antimalarial drug for treating Plasmodium falciparum malaria. To produce specific antibodies to artesunate, the carboxyl group of artesunate was directly conjugated to carrier protein as the immunogen. A specific monoclonal antibody (mAb) 3D82G6 against artesunate was obtained by high-throughput screening of positive hybridoma clones. This monoclonal antibody had 4.0, 0.5, and 0.9 % cross reactivities with artemisinin, dihydroartemisinin, and artemether, respectively. A dipstick immunoassay was developed, and the indicator range for artesunate was 1000-2000 ng mL(-1). No interference was observed with artemisinin, dihydroartemisinin, artemether, and other commonly used antimalarial drugs for up to 20,000 ng mL(-1). The dipsticks were used for determination of artesunate contents in commercial drugs, and the results were agreeable with those determined by high-performance liquid chromatography. This dipstick, with its specificity and sensitivity for artesunate and simplicity to use, makes it a potential point-of-care device for rapid quality evaluation of artesunate-containing antimalarial drugs. Graphical Abstract Specific monoclonal antibody-based lateral flow dipstick for artesunate.
青蒿琥酯是治疗恶性疟原虫疟疾的一线抗疟药物。为制备针对青蒿琥酯的特异性抗体，将青蒿琥酯的羧基直接与载体蛋白偶联作为免疫原。通过对阳性杂交瘤克隆进行高通量筛选，获得了一种针对青蒿琥酯的特异性单克隆抗体（mAb）3D82G6。该单克隆抗体与青蒿素、双氢青蒿素和蒿甲醚的交叉反应率分别为4.0%、0.5%和0.9%。开发了一种试纸条免疫分析法，青蒿琥酯的检测范围为1000 - 2000 ng mL⁻¹。对于浓度高达20,000 ng mL⁻¹的青蒿素、双氢青蒿素、蒿甲醚和其他常用抗疟药物，未观察到干扰。该试纸条用于测定市售药品中青蒿琥酯的含量，结果与高效液相色谱法测定的结果一致。这种试纸条对青蒿琥酯具有特异性和敏感性，且使用简便，使其成为一种用于快速质量评估含青蒿琥酯抗疟药物的潜在即时检测设备。图形摘要 基于特异性单克隆抗体的青蒿琥酯侧流试纸条。
