Kharazian B, Hadipour N L, Ejtehadi M R
Department of Chemistry, Tarbiat Modares University, Tehran, Iran.
Department of Chemistry, Tarbiat Modares University, P.O. Box 14115-175, Tehran, Iran.
Int J Biochem Cell Biol. 2016 Jun;75:162-74. doi: 10.1016/j.biocel.2016.02.008. Epub 2016 Feb 9.
Nanoparticles (NP) have capability to adsorb proteins from biological fluids and form protein layer, which is called protein corona. As the cell sees corona coated NPs, the protein corona can dictate biological response to NPs. The composition of protein corona is varied by physicochemical properties of NPs including size, shape, surface chemistry. Processing of protein adsorption is dynamic phenomena; to that end, a protein may desorb or leave a surface vacancy that is rapidly filled by another protein and cause changes in the corona composition mainly by the Vroman effect. In this review, we discuss the interaction between NP and proteins and the available techniques for identification of NP-bound proteins. Also we review current developed computational methods for understanding the NP-protein complex interactions.
纳米颗粒（NP）能够从生物流体中吸附蛋白质并形成蛋白质层，这被称为蛋白质冠层。当细胞识别被冠层包裹的纳米颗粒时，蛋白质冠层能够决定纳米颗粒的生物学反应。蛋白质冠层的组成会因纳米颗粒的物理化学性质（包括大小、形状、表面化学性质）而有所不同。蛋白质吸附过程是一种动态现象；为此，一种蛋白质可能会解吸或留下一个表面空位，该空位会迅速被另一种蛋白质填充，主要通过弗罗曼效应导致冠层组成发生变化。在这篇综述中，我们讨论了纳米颗粒与蛋白质之间的相互作用以及用于鉴定与纳米颗粒结合的蛋白质的现有技术。我们还综述了当前为理解纳米颗粒 - 蛋白质复合物相互作用而开发的计算方法。
