Xu Wei, Zhang Wenli, Zhang Tao, Jiang Bo, Mu Wanmeng
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, China.
Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, 214122, China.
Appl Microbiol Biotechnol. 2016 Apr;100(7):2985-92. doi: 10.1007/s00253-016-7369-z. Epub 2016 Feb 15.
L-Rhamnose isomerase (L-RI, EC 5.3.1.14), catalyzing the isomerization between L-rhamnose and L-rhamnulose, plays an important role in microbial L-rhamnose metabolism and thus occurs in a wide range of microorganisms. It attracts more and more attention because of its broad substrate specificity and its great potential in enzymatic production of various rare sugars. In this article, the enzymatic properties of various reported L-RIs were compared in detail, and their applications in the production of L-rhamnulose and various rare sugars including D-allose, D-gulose, L-lyxose, L-mannose, L-talose, and L-galactose were also reviewed.
L-鼠李糖异构酶（L-RI，EC 5.3.1.14）催化L-鼠李糖和L-鼠李酮糖之间的异构化反应，在微生物L-鼠李糖代谢中发挥重要作用，因此广泛存在于多种微生物中。由于其广泛的底物特异性以及在各种稀有糖的酶促生产中具有巨大潜力，它越来越受到关注。在本文中，详细比较了各种已报道的L-RI的酶学性质，并对它们在L-鼠李酮糖以及包括D-阿洛糖、D-古洛糖、L-来苏糖、L-甘露糖、L-塔罗糖和L-半乳糖在内的各种稀有糖生产中的应用进行了综述。
