McCaffery R E, Coggins L W, Doherty I, Kennedy I, O'Prey M, McColl L, Campo M S
Beatson Institute for Cancer Research, Bearsden, Glasgow, Scotland.
Oncogene. 1989 Dec;4(12):1441-8.
Three different Harvey-ras (Ha-ras) genes have been identified in the bovine genome by screening a lambda phage-bovine DNA library with the ras gene of the Harvey murine sarcoma virus. The genes have been characterized by hybridization and heteroduplex analysis with both the viral and human Ha-ras genes. Based on heteroduplex mapping, the putative direction of transcription and the approximate position of the coding regions of the bovine genes were established. Two genes, bovine Ha-ras 1 and 2, both show an arrangement of exons and introns typical of c-Ha-ras genes. However, they are distinct entities as they have different nucleotide sequences and physical maps, and heteroduplexes between them contain a region of non-homology upstream of exon 1. The third gene, c-Ha-ras 3, does not contain introns and is a pseudogene, analogous to human c-Ha-ras 2. The nucleotide sequence of both Ha-ras 1 and Ha-ras 2 has been determined and shows that Ha-ras 1 encodes a bona fide ras protein, whereas Ha-ras 2 has diverged considerably from a recognizably functional sequence.
通过用哈维鼠肉瘤病毒的ras基因筛选λ噬菌体 - 牛DNA文库，在牛基因组中鉴定出了三种不同的哈维 - ras（Ha - ras）基因。通过与病毒和人类Ha - ras基因进行杂交和异源双链分析，对这些基因进行了表征。基于异源双链图谱，确定了牛基因转录的推定方向和编码区的大致位置。两个基因，即牛Ha - ras 1和2，都显示出典型的c - Ha - ras基因的外显子和内含子排列。然而，它们是不同的实体，因为它们具有不同的核苷酸序列和物理图谱，并且它们之间的异源双链在外显子1上游包含一个非同源区域。第三个基因，c - Ha - ras 3，不包含内含子，是一个假基因，类似于人类c - Ha - ras 2。已经确定了Ha - ras 1和Ha - ras 2的核苷酸序列，结果表明Ha - ras 1编码一种真正的ras蛋白，而Ha - ras 2已经与可识别的功能序列有很大差异。
