Lee J S, Choe J, Park E H
Department of Biology, College of Natural Sciences, Hanyang University, Seoul, Korea.
Biochem Mol Biol Int. 1995 Jan;35(1):57-63.
The ras homologue of the rivulid fish Rivulus marmoratus was isolated and characterized by screening about 3.0 x 10(5) genomic clones from a Rivulus genomic library using human c-Ha-ras probe. When this clone was partially sequenced focusing on the region coding exons, it showed 97.5% amino acid homology to the human c-Ki-ras gene. The Rivulus c-Ki-ras gene spans about 6.3 kb and consists of five exons including the alternative splicing exon 4a/4b. The exon-intron boundaries of Rivulus c-Ki-ras gene coincided with the GT/AG rule of consensus splice acceptor and donor sequences as in mammalian c-Ki-ras genes. Amino acid sequence analysis of some domain regions of the Rivulus c-Ki-ras gene revealed 100% identity to mammalian c-Ki-ras gene. This report is the first that elucidate the entire structure of c-Ki-ras in a fish.
通过使用人c-Ha-ras探针筛选来自新月鱼基因组文库的约3.0×10⁵个基因组克隆，分离并鉴定了新月鱼（Rivulus marmoratus）的ras同源物。当对该克隆进行部分测序并聚焦于编码外显子的区域时，它与人类c-Ki-ras基因显示出97.5%的氨基酸同源性。新月鱼c-Ki-ras基因跨度约6.3 kb，由五个外显子组成，包括可变剪接外显子4a/4b。新月鱼c-Ki-ras基因的外显子-内含子边界与哺乳动物c-Ki-ras基因一样，符合共有剪接受体和供体序列的GT/AG规则。对新月鱼c-Ki-ras基因一些结构域区域的氨基酸序列分析显示与哺乳动物c-Ki-ras基因具有100%的同一性。本报告首次阐明了鱼类中c-Ki-ras的完整结构。
