Yu Changning, Lu Peng, Liu Shangxi, Li Qiao, Xu Erhua, Gong Joshua, Liu Song, Yang Chengbo
Department of Biosystems Engineering, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada.
Department of Animal Science, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada.
ACS Omega. 2021 Mar 19;6(12):8382-8393. doi: 10.1021/acsomega.1c00117. eCollection 2021 Mar 30.
Deoxynivalenol (DON) contamination occurs in feeds and causes a reduction in growth performance, damage to the intestinal epithelial cells, and increased susceptibility to enteric pathogen challenge. Sodium metabisulfite (SMBS) has shown promise in reducing DON; however, SMBS quickly degrades under aqueous acidic conditions such as the environment within a stomach. Thus, protection of SMBS is required for effective delivery to the small intestine to detoxify DON. This study was to encapsulate SMBS into hydrogenated palm oil-based microparticles for its delivery to the small intestine and to evaluate its efficacy on DON detoxification in simulated intestinal fluids using IPEC-J2 cells . The diameter of the SMBS containing microparticles was 511 ± 135 μm, and the loading capacity of SMBS in the microparticles was 45.50%; 1.41% of the encapsulated SMBS (ES) was released into the simulated gastric fluid, and 66.39% of ES was progressively released into the simulated intestinal fluid within 4 h at 37 °C. In IPEC-J2 cells, when DON was treated with the simulated gastric fluid containing 0.5% ES for 2 h, then mixed with the simulated intestinal fluid (1:1) and incubated for 2 h, cytotoxicity was not observed. DON treated with 0.5 ES decreased the gene expression of inflammatory cytokines in the cells compared with DON alone and maintained the cell integrity. To conclude, the SMBS containing microparticles were stable in the simulated gastric fluid and allowed a progressive release of SMBS in the simulated intestinal fluid. The released SMBS in the simulated intestinal fluid effectively detoxified DON.
脱氧雪腐镰刀菌烯醇（DON）污染存在于饲料中，会导致生长性能下降、肠道上皮细胞受损以及对肠道病原体攻击的易感性增加。焦亚硫酸钠（SMBS）在降低DON方面已显示出前景；然而，SMBS在酸性水条件下（如胃内环境）会迅速降解。因此，需要对SMBS进行保护，以便有效地输送到小肠以解毒DON。本研究旨在将SMBS包封到氢化棕榈油基微粒中，将其输送到小肠，并使用IPEC-J2细胞评估其在模拟肠液中对DON解毒的功效。含SMBS微粒的直径为511±135μm，SMBS在微粒中的负载量为45.50%；1.41%的包封SMBS（ES）释放到模拟胃液中，66.39%的ES在37℃下4小时内逐渐释放到模拟肠液中。在IPEC-J2细胞中，当DON用含0.5%ES的模拟胃液处理2小时，然后与模拟肠液（1:1）混合并孵育2小时时，未观察到细胞毒性。与单独的DON相比，用0.5%ES处理的DON降低了细胞中炎性细胞因子的基因表达，并维持了细胞完整性。总之，含SMBS的微粒在模拟胃液中稳定，并允许SMBS在模拟肠液中逐渐释放。在模拟肠液中释放的SMBS有效地解毒了DON。
