Sugano Shoji, Hayashi Nagao, Kawagoe Yasushi, Mochizuki Susumu, Inoue Haruhiko, Mori Masaki, Nishizawa Yoko, Jiang Chang-Jie, Matsui Minami, Takatsuji Hiroshi
Disease Resistant Crops Research Unit, GMO Center, National Institute of Agrobiological Sciences (NIAS), Kannondai 2-1-2, Tsukuba, Ibaraki, 305-8602, Japan.
Functional Plant Research Unit, Division of Plant Sciences, National Institute of Agrobiological Sciences (NIAS), Kannondai 2-1-2, Tsukuba, Ibaraki, 305-8602, Japan.
Plant Mol Biol. 2016 May;91(1-2):81-95. doi: 10.1007/s11103-016-0444-0. Epub 2016 Feb 15.
Membrane trafficking plays pivotal roles in many cellular processes including plant immunity. Here, we report the characterization of OsVAMP714, an intracellular SNARE protein, focusing on its role in resistance to rice blast disease caused by the fungal pathogen Magnaporthe oryzae. Disease resistance tests using OsVAMP714 knockdown and overexpressing rice plants demonstrated the involvement of OsVAMP714 in blast resistance. The overexpression of OsVAMP7111, whose product is highly homologous to OsVAMP714, did not enhance blast resistance to rice, implying a potential specificity of OsVAMP714 to blast resistance. OsVAMP714 was localized to the chloroplast in mesophyll cells and to the cellular periphery in epidermal cells of transgenic rice plant leaves. We showed that chloroplast localization is critical for the normal OsVAMP714 functioning in blast resistance by analyzing the rice plants overexpressing OsVAMP714 mutants whose products did not localize in the chloroplast. We also found that OsVAMP714 was located in the vacuolar membrane surrounding the invasive hyphae of M. oryzae. Furthermore, we showed that OsVAMP714 overexpression promotes leaf sheath elongation and that the first 19 amino acids, which are highly conserved between animal and plant VAMP7 proteins, are crucial for normal rice plant growths. Our studies imply that the OsVAMP714-mediated trafficking pathway plays an important role in rice blast resistance as well as in the vegetative growth of rice.
膜泡运输在包括植物免疫在内的许多细胞过程中起着关键作用。在此，我们报道了一种细胞内SNARE蛋白OsVAMP714的特性，重点研究了其在抵抗由真菌病原体稻瘟病菌引起的水稻稻瘟病中的作用。使用OsVAMP714基因敲低和过表达水稻植株进行的抗病性测试表明，OsVAMP714参与了稻瘟病抗性。OsVAMP7111的产物与OsVAMP714高度同源，其过表达并未增强水稻对稻瘟病的抗性，这意味着OsVAMP714对稻瘟病抗性可能具有特异性。在转基因水稻植株叶片中，OsVAMP714定位于叶肉细胞的叶绿体和表皮细胞的细胞周边。通过分析过表达其产物不在叶绿体中定位的OsVAMP714突变体的水稻植株，我们表明叶绿体定位对于OsVAMP714在稻瘟病抗性中的正常功能至关重要。我们还发现OsVAMP714位于稻瘟病菌侵入菌丝周围的液泡膜上。此外，我们表明OsVAMP714过表达促进叶鞘伸长，并且动物和植物VAMP7蛋白之间高度保守的前19个氨基酸对于水稻植株的正常生长至关重要。我们的研究表明，OsVAMP714介导的运输途径在水稻稻瘟病抗性以及水稻营养生长中起着重要作用。
