Matt Katja, Burger Katharina, Gebhard Daniel, Bergemann Jörg
Department of Life Sciences, Albstadt-Sigmaringen University of Applied Sciences, Sigmaringen, Germany.
Department of Life Sciences, Albstadt-Sigmaringen University of Applied Sciences, Sigmaringen, Germany.
Mech Ageing Dev. 2016 Mar;154:24-9. doi: 10.1016/j.mad.2016.02.008. Epub 2016 Feb 13.
Caloric restrictive feeding prolongs the lifespan of a variety of model organisms like rodents and invertebrates. It has been shown that caloric restriction reduces age-related as well as overall-mortality, reduces oxidative stress and influences DNA repair ability positively. There are numerous studies underlining this, but fewer studies involving humans exist. To contribute to a better understanding of the correlation of calorie reduction and DNA repair in humans, we adapted the host cell reactivation assay to an application with human peripheral blood mononuclear cells. Furthermore, we used this reliable and reproducible assay to research the influence of a special kind of calorie reduction, namely F. X. Mayr therapy, on DNA repair capacity. We found a positive effect in all persons with low pre-existing DNA repair capacity. In individuals with normal pre-existing DNA repair capacity, no effect on DNA repair capacity was detectable. Decline of DNA repair, accumulation of oxidative DNA damages, mitochondrial dysfunction, telomere shortening as well as caloric intake are widely thought to contribute to aging. With regard to that, our results can be considered as a strong indication that calorie reduction may support DNA repair processes and thus contribute to a healthier aging.
热量限制喂养可延长多种模式生物的寿命，如啮齿动物和无脊椎动物。研究表明，热量限制可降低与年龄相关的死亡率以及总体死亡率，减少氧化应激，并对DNA修复能力产生积极影响。有大量研究证实了这一点，但涉及人类的研究较少。为了更好地理解人类热量减少与DNA修复之间的相关性，我们将宿主细胞再激活试验应用于人类外周血单核细胞。此外，我们使用这种可靠且可重复的试验来研究一种特殊的热量减少方式，即F.X.迈尔疗法对DNA修复能力的影响。我们发现，对于所有预先存在低DNA修复能力的人都有积极作用。在预先存在正常DNA修复能力的个体中，未检测到对DNA修复能力的影响。DNA修复能力下降、氧化性DNA损伤积累、线粒体功能障碍、端粒缩短以及热量摄入，普遍被认为与衰老有关。就此而言，我们的结果可被视为一个有力的迹象，表明热量减少可能支持DNA修复过程，从而有助于更健康地衰老。
