Matt Katja, Hochecker Barbara, Schöller-Mann Alica, Bergemann Jörg
Department of Life Sciences, Albstadt-Sigmaringen University of Applied Sciences, Sigmaringen, Germany.
Nutr Health. 2020 Sep;26(3):253-262. doi: 10.1177/0260106020932732. Epub 2020 Jun 18.
Finding ways to a healthier ageing are increasingly becoming the focus of geriatric research. One way to accomplish this could be calorie restriction, as this is known to positively influence the ageing of model organisms.
The aim of this study was to investigate the influence of calorie reduction (F. X. Mayr therapy) and of the calorie restriction mimetics resveratrol and spermidine on the expression of ageing-associated genes.
mRNA expression in peripheral blood mononuclear cells (PBMCs) of 18 participants taking part in an F. X. Mayr therapy was analysed. The PBMCs of one additional participant were treated with spermidine or resveratrol. mRNA expression of SIRT1, SIRT3, FOXO3 and SOD2 was determined for these two calorie restriction mimetics. For the F. X. Mayr therapy samples, mRNA of XPA was analysed additionally.
mRNA expression of the ageing-associated genes showed a distinct donor variation during F. X. Mayr therapy, with a significant increase in mRNA expression of SIRT1. Expression of XPA was similar to SIRT1, with a significant correlation at the last time point tested. Spermidine treatment of PBMCs resulted in a significantly increased expression of all genes tested, whereas resveratrol treatment caused a significant increase of SIRT3, FOXO3 and SOD2 mRNA expression.
By increasing SIRT1 and XPA mRNA expression, calorie reduction in the form of F. X. Mayr therapy could contribute to a healthier ageing; however, the donor variability observed showed that not everyone benefited from this. Calorie restriction mimetics may be an option for promote healthier ageing for those who do not benefit from calorie reduction.
寻找实现更健康衰老的方法日益成为老年医学研究的焦点。实现这一目标的一种方法可能是热量限制，因为已知其对模式生物的衰老有积极影响。
本研究旨在调查热量减少（F.X. 迈尔疗法）以及热量限制模拟物白藜芦醇和亚精胺对衰老相关基因表达的影响。
分析了18名参与F.X. 迈尔疗法的参与者外周血单个核细胞（PBMC）中的mRNA表达。另外一名参与者的PBMC用亚精胺或白藜芦醇进行处理。测定了这两种热量限制模拟物的SIRT1、SIRT3、FOXO3和SOD2的mRNA表达。对于F.X. 迈尔疗法样本，还分析了XPA的mRNA。
在F.X. 迈尔疗法期间，衰老相关基因的mRNA表达显示出明显的个体差异，SIRT1的mRNA表达显著增加。XPA的表达与SIRT1相似，在最后测试时间点有显著相关性。PBMC用亚精胺处理导致所有测试基因的表达显著增加，而白藜芦醇处理导致SIRT3、FOXO3和SOD2的mRNA表达显著增加。
通过增加SIRT1和XPA的mRNA表达，F.X. 迈尔疗法形式的热量减少可能有助于实现更健康的衰老；然而，观察到的个体差异表明并非每个人都能从中受益。对于那些无法从热量减少中受益的人，热量限制模拟物可能是促进更健康衰老的一种选择。
