Souza Adelmo S, Nunes Luiz A O, Silva Ivan G N, Oliveira Fernando A M, da Luz Leonis L, Brito Hermi F, Felinto Maria C F C, Ferreira Rute A S, Júnior Severino A, Carlos Luís D, Malta Oscar L
Departamento de Química Fundamental-CCEN-UFPE, Cidade Universitária, Recife-PE, 50670-901, Brazil.
Instituto de Física de São Carlos, Universidade de São Paulo - USP, CEP 13560-970, São Carlos, SP, Brazil.
Nanoscale. 2016 Mar 7;8(9):5327-33. doi: 10.1039/c6nr00158k.
Temperature measurements ranging from a few degrees to a few hundreds of Kelvin are of great interest in the fields of nanomedicine and nanotechnology. Here, we report a new ratiometric luminescent thermometer using thermally excited state absorption of the Eu(3+) ion. The thermometer is based on the simple Eu(3+) energy level structure and can operate between 180 and 323 K with a relative sensitivity ranging from 0.7 to 1.7% K(-1). The thermometric parameter is defined as the ratio between the emission intensities of the (5)D0 → (7)F4 transition when the (5)D0 emitting level is excited through the (7)F2 (physiological range) or (7)F1 (down to 180 K) level. Nano and microcrystals of Y2O3:Eu(3+) were chosen as a proof of concept of the operational principles in which both excitation and detection are within the first biological transparent window. A novel and of paramount importance aspect is that the calibration factor can be calculated from the Eu(3+) emission spectrum avoiding the need for new calibration procedures whenever the thermometer operates in different media.
从几开尔文到几百开尔文的温度测量在纳米医学和纳米技术领域具有重大意义。在此，我们报告一种利用铕（Eu(3+)）离子热激发态吸收的新型比率发光温度计。该温度计基于简单的Eu(3+)能级结构，可在180至323 K之间运行，相对灵敏度范围为0.7%至1.7% K(-1)。测温参数定义为当(5)D0发射能级通过(7)F2（生理范围）或(7)F1（低至180 K）能级被激发时，(5)D0 → (7)F4跃迁发射强度之间的比率。选择Y2O3:Eu(3+)的纳米和微晶作为操作原理的概念验证，其中激发和检测均在第一个生物透明窗口内。一个新颖且至关重要的方面是，校准因子可从Eu(3+)发射光谱计算得出，从而避免了温度计在不同介质中运行时需要新的校准程序。
