Verma Shailender Kumar, Kumar Satish, Sheikh Imran, Malik Sachin, Mathpal Priyanka, Chugh Vishal, Kumar Sundip, Prasad Ramasare, Dhaliwal Harcharan Singh
a School of Life Sciences , Central University of Himachal Pradesh , Dharamshala, Kangra , Himachal Pradesh ;
b Department of Biotechnology , Indian Institute of Technology Roorkee , Roorkee , Uttarakhand ;
Int J Radiat Biol. 2016;92(3):132-9. doi: 10.3109/09553002.2016.1135263. Epub 2016 Feb 17.
To transfer the 2S chromosomal fragment(s) of Aegilops kotschyi (2S(k)) into the bread wheat genome which could lead to the biofortification of wheat with high grain iron and zinc content.
Wheat-Ae. kotschyi 2A/2S(k) substitution lines with high grain iron and zinc content were used to transfer the gene/loci for high grain Fe and Zn content into wheat using seed irradiation approach.
Bread wheat plants derived from 40 krad-irradiated seeds showed the presence of univalents and multivalents during meiotic metaphase-I. Genomic in situ hybridization analysis of seed irradiation hybrid F2 seedlings showed several terminal and interstitial signals indicated the introgression of Ae. kotschyi chromosome segments. This proves the efficacy of seed radiation hybrid approach in gene transfer experiments. All the radiation-treated hybrid plants with high grain Fe and Zn content were analyzed with wheat group 2 chromosome-specific polymorphic simple sequence repeat markers to identify the introgression of small alien chromosome fragment(s).
Radiation-induced hybrids showed more than 65% increase in grain iron and 54% increase in Zn contents with better harvest index than the elite wheat cultivar WL711 indicating effective and compensating translocations of 2S(k) fragments into wheat genome.
将节节麦（Aegilops kotschyi）的2S染色体片段导入普通小麦基因组，以实现小麦籽粒铁和锌含量的生物强化。
利用籽粒铁和锌含量高的小麦-节节麦2A/2S(k)代换系，通过种子辐照方法将籽粒铁和锌含量高的基因/位点导入小麦。
来自40千拉德辐照种子的普通小麦植株在减数分裂中期I出现单价体和多价体。对种子辐照杂种F2幼苗进行基因组原位杂交分析，发现多个末端和居间信号，表明节节麦染色体片段已渗入。这证明了种子辐射杂种方法在基因转移实验中的有效性。用小麦第2组染色体特异性多态性简单序列重复标记对所有经辐射处理的、籽粒铁和锌含量高的杂种植株进行分析，以鉴定小的外源染色体片段的渗入情况。
辐射诱导的杂种籽粒铁含量增加超过65%，锌含量增加54%，收获指数优于优良小麦品种WL711，表明2S(k)片段有效且补偿性地易位到小麦基因组中。
