Transfer of HMW glutenin subunits from to wheat through radiation hybridization.

High molecular weight glutenin subunits (HMWGS) are responsible for dough elasticity and bread making quality of bread wheat. Related wild non-progenitor species, possesses higher molecular weight and glutenin subunits than the bread wheat cultivars. A wheat- substitution line with 1U chromosome was used for the transfer of (HMWGS) of 1U to wheat by using pollen radiation hybridization approach. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiling showed different patterns of allelic variations with either the presence or absence of HMWGS, - (1, null), - (7, 7 + 8, 17 + 18) and - (5 + 10, 2 + 12, null). The pollen irradiated wheat- derivatives, B-56-1-4-2, B-56-1-4-3, B-14-1 and B-14-2 with 1Ux and 1Uy and absence or presence of some - and - HMWGS showed high micro SDS sedimentation test (MST) values while B-16-1 and B-16-2 had moderate MST values and high protein content. However, B-58-3 with transfer of -1Ux + 1Uy for - showed very low MST values indicating that -1Ux + 1Uy enhance MST value only in the presence of HMWGS. The transfer/substitution of alien HMW-GS for - and or - loci only can lead to improved bread making quality of wheat.