Plantaz D, Goullier A, Jouk P S, Bost M
Service de Médecine Infantile, Centre Hospitalier Régional, Grenoble, France.
Pediatrie. 1987;42(5):387-91.
An IgM immunosorbent agglutination assay (ISAGA) test was evaluated for detection of IgM antibodies in diagnosis of congenital toxoplasma infection, and compared with IgM IFI test. Of 36 sera from infants with congenital infection, the IgM ISAGA test was positive in 19 cases (52%), whereas the IgM IFI test was positive in only 4 cases (11%). Of those sera obtained during the first 90 days of life from the infected infants, 70% were positive in the IgM ISAGA test whereas only 23% in the IgM IFI test. Neonatal sera from 10 infants suspected, but in whom this diagnosis was ruled out, were also evaluated: one false positive was found with the ISAGA test, but without control 15 days later. The ISAGA test is a simple, sensitive and probably highly specific test for diagnosis of congenital toxoplasma infection.
对一种IgM免疫吸附凝集试验（ISAGA）进行了评估，以检测先天性弓形虫感染诊断中的IgM抗体，并与IgM免疫荧光试验（IFI）进行比较。在36份先天性感染婴儿的血清中，IgM ISAGA试验有19例呈阳性（52%），而IgM IFI试验仅4例呈阳性（11%）。在感染婴儿出生后头90天内获得的那些血清中，70%的IgM ISAGA试验呈阳性，而IgM IFI试验仅23%呈阳性。还对10例疑似但排除该诊断的婴儿的新生儿血清进行了评估：ISAGA试验发现1例假阳性，但15天后未进行对照检测。ISAGA试验是一种用于诊断先天性弓形虫感染的简单、灵敏且可能高度特异的试验。
