通过液滴数字PCR检测遗传性染色体整合HHV-6A的造血细胞移植受者中人类疱疹病毒6B（HHV-6B）的再激活情况。
Detection of Human Herpesvirus 6B (HHV-6B) Reactivation in Hematopoietic Cell Transplant Recipients with Inherited Chromosomally Integrated HHV-6A by Droplet Digital PCR.
作者信息
Sedlak Ruth Hall, Hill Joshua A, Nguyen Thuy, Cho Michelle, Levin Greg, Cook Linda, Huang Meei-Li, Flamand Louis, Zerr Danielle M, Boeckh Michael, Jerome Keith R
机构信息
Department of Laboratory Medicine, University of Washington, Seattle, Washington, USA.
Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.
出版信息
J Clin Microbiol. 2016 May;54(5):1223-7. doi: 10.1128/JCM.03275-15. Epub 2016 Feb 17.
Abstract
The presence of inherited chromosomally integrated human herpesvirus 6 (ciHHV-6) in hematopoietic cell transplant (HCT) donors or recipients confounds molecular testing for HHV-6 reactivation, which occurs in 30 to 50% of transplants. Here we describe a multiplex droplet digital PCR clinical diagnostic assay that concurrently distinguishes between HHV-6 species (A or B) and identifies inherited ciHHV-6. By applying this assay to recipient post-HCT plasma and serum samples, we demonstrated reactivation of HHV-6B in 25% (4/16 recipients) of HCT recipients with donor- or recipient-derived inherited ciHHV-6A, underscoring the need for diagnostic testing for HHV-6 infection even in the presence of ciHHV-6.
摘要
造血细胞移植（HCT）供者或受者中存在遗传性染色体整合的人疱疹病毒6型（ciHHV-6），这会干扰HHV-6再激活的分子检测，HHV-6再激活发生在30%至50%的移植中。在此，我们描述了一种多重液滴数字PCR临床诊断检测方法，该方法可同时区分HHV-6的种类（A或B）并鉴定遗传性ciHHV-6。通过将该检测方法应用于HCT受者的移植后血浆和血清样本，我们证明了在25%（4/16例受者）具有供体或受者来源的遗传性ciHHV-6A的HCT受者中，HHV-6B发生了再激活，这突出表明即使存在ciHHV-6，也需要对HHV-6感染进行诊断检测。
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