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冷应激对大鼠免疫力的影响。

Effect of cold stress on immunity in rats.

作者信息

Hu Guo-Zhu, Yang Si-Jun, Hu Wei-Xu, Wen Zhu, He Dan, Zeng Li-Feng, Xiang Qin, Wu Xiao-Mu, Zhou Wen-Yun, Zhu Qing-Xian

机构信息

Institute of Clinical Medical Sciences, Jiangxi Province People's Hospital, Nanchang, Jiangxi 330006, P.R. China.

Graduate Student Department, Medical College of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.

出版信息

Exp Ther Med. 2016 Jan;11(1):33-42. doi: 10.3892/etm.2015.2854. Epub 2015 Nov 11.

Abstract

An increase in the morbidity of upper respiratory tract infections and the attack and exacerbation of autoimmune diseases has been observed to occur in the few days following sudden environmental temperature decreases, but the mechanisms for these phenomena are not well understood. To determine the effect of a sudden ambient temperature drop on the levels of stress hormones and T-lymphocyte cytokines in the plasma, the Toll-like receptor 4 (TLR4) expression of immunocompetent cells in rat spleens and the levels of regulatory T (Treg) cells in the peripheral blood, Sprague Dawley rats were divided into three groups of different ambient temperatures (20, 4 and -12°C). In each group, there were four observation time-points (1, 12, 24 and 48 h). Each ambient temperature group was subdivided into non-stimulation, lipopolysaccharide-stimulation and concanavalin A-stimulation groups. The levels of adrenocorticotropin (ACTH), epinephrine (EPI), angiotensin-II (ANG-II), interleukin-2 (IL-2), interferon-γ (IFN-γ), IL-4 and IL-10 in the plasma were determined using ELISA. The cellular expression levels of TLR4 and the presence of cluster of differentiation (CD)4CD25 and CD4CD25Forkhead box P3 (Foxp3) cells were determined using flow cytometry. The experiments demonstrated that the ACTH, EPI, ANG-II and IL-10 levels in the plasma were significantly increased at 4 and -12°C compared with those at 20°C, while the plasma levels of IFN-γ, IL-2 and IL-4, the TLR4 expression rates of immunocompetent cells in the rat spleen and the percentage of CD4CD25Foxp3 Treg cells among the CD4CD25 Treg cells in the peripheral blood were decreased at 4 and -12°C compared with those at 20°C. These data indicate that cold stress affects the stress hormones and the innate and adaptive immunity functions in rats.

摘要

据观察,在环境温度突然下降后的几天内,上呼吸道感染的发病率以及自身免疫性疾病的发作和加重情况会增加,但这些现象的机制尚未完全明确。为了确定环境温度突然下降对血浆中应激激素和T淋巴细胞细胞因子水平、大鼠脾脏中免疫活性细胞的Toll样受体4(TLR4)表达以及外周血中调节性T(Treg)细胞水平的影响,将Sprague Dawley大鼠分为三组,置于不同的环境温度(20、4和-12°C)下。每组设置四个观察时间点(1、12、24和48小时)。每个环境温度组又细分为非刺激组、脂多糖刺激组和伴刀豆球蛋白A刺激组。采用酶联免疫吸附测定法(ELISA)测定血浆中促肾上腺皮质激素(ACTH)、肾上腺素(EPI)、血管紧张素-II(ANG-II)、白细胞介素-2(IL-2)、干扰素-γ(IFN-γ)、IL-4和IL-10的水平。使用流式细胞术测定TLR4的细胞表达水平以及分化簇(CD)4CD25和CD4CD25叉头框蛋白P3(Foxp3)细胞的存在情况。实验表明,与20°C相比,4°C和-12°C时血浆中的ACTH、EPI、ANG-II和IL-10水平显著升高,而4°C和-12°C时血浆中的IFN-γ、IL-2和IL-4水平、大鼠脾脏中免疫活性细胞的TLR4表达率以及外周血中CD4CD25 Treg细胞中CD4CD25Foxp3 Treg细胞的百分比均低于20°C时的水平。这些数据表明,冷应激会影响大鼠的应激激素以及先天性和适应性免疫功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6fd7/4726882/762858130038/etm-11-01-0033-g00.jpg

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