Regional Plant Resource Centre, Nayapalli, Bhubaneswar 751015, Odisha, India.
Centre for Energy, Indian Institute of Technology, Guwahati 781039, Assam, India; Department of Chemical Engineering, Indian Institute of Technology, Guwahati 781039, Assam, India.
Bioresour Technol. 2016 May;207:205-12. doi: 10.1016/j.biortech.2016.01.118. Epub 2016 Feb 15.
Protocols were developed for efficient release of glucose from the biomass of Senna siamea, one of the highly efficient biomass producing tree legumes. Composition of mature, 1year and 2years coppice biomass were analysed. For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan. Of the biomass of different particle sizes (40-200mesh) used for saccharification, 40-60mesh shown the maximum glucose release. COSLIF pretreated mature, 1year and 2years coppice biomass showed equivalent glucose release profiles.
制定了从塞内西亚米亚生物质中高效释放葡萄糖的方案，塞内西亚米亚是一种高效生产生物质的豆科树种之一。分析了成熟、1 年生和 2 年生萌蘖生物质的组成。为了水解葡聚糖，使用了两种预处理方法，即纤维素溶剂和有机溶剂基木质纤维素分级（COSLIF）和碱（氢氧化钠）预处理；COSLIF（85%磷酸，50°C 孵育 45min）预处理成熟生物质在使用 5 个滤纸单位（FPU）纤维素酶和 10 个国际单位（IU）β-葡萄糖苷酶/克葡聚糖孵育 72 小时后，表现出最佳效果，释放了 88.90%的葡萄糖。在用于糖化的不同粒径（40-200 目）的生物质中，40-60 目显示出最大的葡萄糖释放。COSLIF 预处理的成熟、1 年生和 2 年生萌蘖生物质显示出等效的葡萄糖释放曲线。
