Baldim Victor, Ismail Abdulghani, Taladriz-Blanco Patricia, Griveau Sophie, de Oliveira Marcelo Ganzarolli, Bedioui Fethi
Institute of Chemistry, University of Campinas , UNICAMP, Campinas, São Paulo, 13083-970, Brazil.
Chimie ParisTech, PSL Research University, Unité de Technologies Chimiques et Biologiques pour la Santé (UTCBS), 75005 Paris, France.
Anal Chem. 2016 Mar 15;88(6):3115-20. doi: 10.1021/acs.analchem.5b04035. Epub 2016 Mar 2.
S-Nitrosothiols (RSNOs) are carriers of nitric oxide (NO) and have important biological activities. We propose here the use of gold nanoparticles (AuNPs) and NO-selective amperometric microsensor for the detection and quantification of S-nitrosoglutathione (GSNO) as a step toward the determination of plasma RSNOs. AuNPs were used to decompose RSNOs with the quantitative release of free NO which was selectively detected with a NO microsensor. The optimal [GSNO]/[AuNPs] ratio was determined, corresponding to an excess of AuNP surface relative to the molar GSNO amount. Moreover, the influence of free plasma thiols on this method was investigated and a protocol based on the blocking of free thiols with iodoacetic acid, forming the carboxymethyl derivative of the cysteine residues, is proposed.
S-亚硝基硫醇（RSNOs）是一氧化氮（NO）的载体，具有重要的生物活性。我们在此提出使用金纳米颗粒（AuNPs）和NO选择性安培微传感器来检测和定量S-亚硝基谷胱甘肽（GSNO），作为测定血浆RSNOs的第一步。AuNPs用于分解RSNOs，定量释放游离NO，然后用NO微传感器进行选择性检测。确定了最佳的[GSNO]/[AuNPs]比例，该比例对应于相对于GSNO摩尔量而言过量的AuNP表面。此外，研究了游离血浆硫醇对该方法的影响，并提出了一种基于用碘乙酸阻断游离硫醇，形成半胱氨酸残基的羧甲基衍生物的方案。
