Chimie ParisTech, PSL Research University, INSERM 1022, CNRS 8258, Paris Descartes, Unité de Technologies Chimiques et Biologiques pour la Santé, 75005 Paris, France.
Instituto de Química, Universidade Estadual de Campinas, UNICAMP, Campinas, SP 13083-970, Brazil.
Analyst. 2018 Dec 17;144(1):180-185. doi: 10.1039/c8an00757h.
S-nitrosothiols (RSNOs) are very important biomolecules that play crucial roles in many physiological and physiopathological processes. They act as NO-donors and are candidates for future medicines. Their identification and quantitation are therefore important for biomedical applications. One, two or more RSNOs can then be combined to design a drug and therefore, the quantification of each is important to establish an acceptable quality control process. Till date, miniaturized devices have been used to detect RSNOs based on their total quantitation without a preceding separation step. This study reports on an original and integrated microdevice allowing for the successive electrokinetic separation of low molecular weight RSNOs, their decomposition under metal catalysis, and their quantitation by amperometric detection of the produced nitrite in the end-channel arrangement, leading to their quantitation in a single run. For this purpose, a commercial SU-8/Pyrex microfluidic system was coupled to a portable and wireless potentiostat. Different operating and running parameters were optimized to achieve the best analytical data, allowing for an LOD equal to 20 μM. The simultaneous separation of S-nitrosoglutathione and S-nitrosocysteine was successfully obtained within 75 s. The proposed methodology using SU-8/Pyrex microfluidic devices opens new possibilities to investigate future drug candidates for NO-donors.
S-亚硝基硫醇（RSNOs）是非常重要的生物分子，在许多生理和病理生理过程中发挥着关键作用。它们作为一氧化氮供体，是未来药物的候选物。因此，它们的鉴定和定量对于生物医学应用非常重要。一个、两个或更多的 RSNO 可以组合设计成一种药物，因此，对每种物质的定量对于建立可接受的质量控制过程非常重要。迄今为止，已经使用微型化设备基于 RSNOs 的总量定量进行检测，而无需进行先前的分离步骤。本研究报告了一种原始的集成微设备，该设备允许在单个运行中连续进行低分子量 RSNOs 的电动分离、在金属催化下的分解，以及通过末端通道排列中产生的亚硝酸盐的电流检测进行定量，从而实现它们的定量。为此，将商业 SU-8/Pyrex 微流控系统与便携式无线电化学工作站耦合。优化了不同的操作和运行参数，以获得最佳的分析数据，使检测限达到 20 μM。在 75 s 内成功实现了 S-亚硝基谷胱甘肽和 S-亚硝基半胱氨酸的同时分离。使用 SU-8/Pyrex 微流控器件的提出的方法为研究未来的一氧化氮供体候选药物开辟了新的可能性。
