Mooranian Armin, Negrulj Rebecca, Al-Salami Hani
Biotechnology & Drug Development Research Laboratory, School of Pharmacy, Curtin Health Innovation Research Institute, Curtin University, Perth, Western Australia, Australia.
Ther Deliv. 2016;7(3):171-8. doi: 10.4155/tde-2015-0001.
Pancreatic β-cell microencapsulation using sodium alginate (SA), polylornithine (PLO) copolymers, and ultrasoluble hydrogels, polystyrenes and polyallamines (PAA), has been heavily studied. However, long-term success remains limited due to poor macrocapsules' physical properties and cell functions. Our study aimed to incorporate percentages of PAA and ursodeoxycholic acid, into SA and PLO dispersion mixture and examine best microencapsulating methods and best macrocapsules containing β-cells.
METHODS/RESULTS: Microencapsulating parameters were examined and the Flow-Vibrational Nozzle built-in system was screened and found to be most efficient at high frequency (1900 Hz). Macrocapsules were produced with or without ursodeoxycholic acid in percentages: 0.018SA:0.01PLO:0.005PAA:0.04ursodeoxycholic acid (up to 100% H2O). Using the refined microencapsulation method with vibrational frequency of 1900 Hz, macrocapsules with ursodeoxycholic acid had optimized cell viability and biological functions and ameliorated inflammatory biomarkers.
High frequency and air-pressure with Flow-Vibrational encapsulation using the mixture: 0.018SA:0.01PLO:0.005PAA:0.04ursodeoxycholic acid resulted in better cell biology suggesting potentials in β-cell transplantation.
使用海藻酸钠（SA）、聚鸟氨酸（PLO）共聚物以及超溶性水凝胶、聚苯乙烯和聚烯丙胺（PAA）对胰腺β细胞进行微囊化已得到大量研究。然而，由于大胶囊的物理性质和细胞功能不佳，长期成功率仍然有限。我们的研究旨在将PAA和熊去氧胆酸的百分比纳入SA和PLO分散混合物中，并研究最佳的微囊化方法以及含有β细胞的最佳大胶囊。
方法/结果：对微囊化参数进行了研究，并对内置流动振动喷嘴系统进行了筛选，发现其在高频（1900赫兹）时效率最高。制备了含有或不含熊去氧胆酸的大胶囊，其比例为：0.018SA:0.01PLO:0.005PAA:0.04熊去氧胆酸（高达100%水）。使用振动频率为1900赫兹的精细微囊化方法，含有熊去氧胆酸的大胶囊具有优化的细胞活力和生物学功能，并改善了炎症生物标志物。
使用0.018SA:0.01PLO:0.005PAA:0.04熊去氧胆酸混合物进行流动振动包封时，高频和气压可产生更好的细胞生物学效果，表明其在β细胞移植方面具有潜力。
