常见水果提取物对有毒胰岛淀粉样多肽淀粉样蛋白的抑制作用。
Inhibition of Toxic IAPP Amyloid by Extracts of Common Fruits.
作者信息
Kao Pei-Yu, Green Evangeline, Pereira Catalina, Ekimura Shauna, Juarez Dennis, Whyte Travis, Arhar Taylor, Malaspina Bianca, Nogaj Luiza A, Moffet David A
机构信息
Department of Chemistry and Biochemistry, Loyola Marymount University, 1 LMU Drive, Los Angeles, CA 90045.
Department of Biology, Mount Saint Mary's College, 12001 Chalon Drive, Los Angeles, CA 90049.
出版信息
J Funct Foods. 2015 Jan 1;12:450-457. doi: 10.1016/j.jff.2014.12.013.
The aggregation of the 37-amino acid polypeptide islet amyloid polypeptide (IAPP, amylin), as either insoluble amyloid or as small oligomers, appears to play a direct role in the death of pancreatic β-islet cells in type 2 diabetes. It is believed that inhibiting the aggregation of IAPP may slow down, if not prevent entirely, the progression of this disease. Extracts of thirteen different common fruits were analyzed for their ability to prevent the aggregation of amyloidogenic IAPP. Thioflavin T binding, immuno-detection and circular dichroism assays were performed to test the inhibitory potential of each extract. Atomic force microscopy was used to visualize the formation of amyloid fibrils with and without each fruit extract. Finally, extracts were tested for their ability to protect living mammalian cells from the toxic effects of amyloid IAPP. Several fruits showed substantial ability to inhibit IAPP aggregation and protect living cells from toxic IAPP amyloid.
由37个氨基酸组成的多肽胰岛淀粉样多肽（IAPP，胰淀素），无论是形成不溶性淀粉样蛋白还是小寡聚体，似乎在2型糖尿病患者胰腺β胰岛细胞死亡中起直接作用。据信，抑制IAPP的聚集可能会减缓（如果不能完全预防的话）这种疾病的进展。分析了13种不同常见水果的提取物抑制淀粉样IAPP聚集的能力。进行硫黄素T结合、免疫检测和圆二色性分析以测试每种提取物的抑制潜力。使用原子力显微镜观察有无每种水果提取物时淀粉样纤维的形成。最后，测试提取物保护活哺乳动物细胞免受淀粉样IAPP毒性作用的能力。几种水果显示出显著的抑制IAPP聚集和保护活细胞免受毒性IAPP淀粉样蛋白影响的能力。
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