Jiang Jian Fa, Sun Ai Jun, Xue Wei, Deng Yan, Wang Yan Fang
Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China.
Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China.
Eur J Obstet Gynecol Reprod Biol. 2016 Apr;199:32-7. doi: 10.1016/j.ejogrb.2016.01.033. Epub 2016 Feb 8.
Adenomyosis is a common gynecologic disease. Alterations in the eutopic endometria might play an important role in the pathogenesis of adenomyosis. Long non-coding RNA (lncRNA) represents key regulators of gene expression. Our goal was to identify differentially expressed long noncoding RNA and messenger RNA (mRNA) in the eutopic endometria of subjects with adenomyosis on a genome-wide scale.
The expression level of lncRNAs and mRNAs in the eutopic endometria from women with adenomyosis and from that of normal control subjects were detected by Affymetrix Human Transcriptome Array 2.0. Bioinformatics analysis was done for further investigation. Three up-regulated and three down-regulated lncRNAs were randomly chosen for validation by quantitative real-time polymerase chain reaction.
A total of 165 lncRNAs and 612 mRNAs were aberrantly expressed in the eutopic endometria of subjects with adenomyosis. Pathway analysis indicated that 40 pathways corresponded to up-regulated transcripts and 39 pathways corresponded to downregulated transcripts. A list of genes that might play roles in the pathogenesis of adenomyosis were produced by comparing the difference between co-expression networks. Expression of six chosen lncRNAs was validated by quantitative real-time polymerase chain reaction.
This study show for the first time that the lncRNA expression profile is altered in women with adenomyosis and provides new biological foundations for further mechanistic studies in this enigmatic disorder.
子宫腺肌病是一种常见的妇科疾病。在位内膜的改变可能在子宫腺肌病的发病机制中起重要作用。长链非编码RNA（lncRNA）是基因表达的关键调节因子。我们的目标是在全基因组范围内鉴定子宫腺肌病患者在位内膜中差异表达的长链非编码RNA和信使RNA（mRNA）。
采用Affymetrix人类转录组阵列2.0检测子宫腺肌病患者和正常对照者在位内膜中lncRNA和mRNA的表达水平。进行生物信息学分析以作进一步研究。随机选择3个上调和3个下调的lncRNA，通过定量实时聚合酶链反应进行验证。
子宫腺肌病患者在位内膜中共有165个lncRNA和612个mRNA异常表达。通路分析表明，40条通路对应上调转录本，39条通路对应下调转录本。通过比较共表达网络之间的差异，列出了可能在子宫腺肌病发病机制中起作用的基因列表。通过定量实时聚合酶链反应验证了6个选定lncRNA的表达。
本研究首次表明子宫腺肌病患者的lncRNA表达谱发生改变，为进一步研究这种疑难病症的发病机制提供了新的生物学基础。
