Li Zenghe, Wang Dan, Yuan Zhiqin, Lu Chao
State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, `100029, China.
Anal Bioanal Chem. 2016 Dec;408(30):8779-8786. doi: 10.1007/s00216-016-9393-3. Epub 2016 Feb 22.
In the last two decades nanoparticles have been widely applied to enhance chemiluminescence (CL). The morphology of nanoparticles has an important influence on nanoparticle-amplified CL. However, studies of nanoparticle-amplified CL focus mainly on the size and shape effects, and no attempt has been made to explore the influence of uniformity in nanoparticle-amplified CL processes. In this study we have investigated nanoparticle uniformity in the luminol-HO CL system using layered double hydroxides (LDHs) as a model material. The results demonstrated that the uniformity of LDHs played a key role in CL amplification. A possible mechanism is that LDHs with high uniformity possess abundant catalytic active sites, which results in high CL intensity. Meanwhile, the sensitivity for HO detection was increased by one order of magnitude (1.0 nM). Moreover, the uniform-LDH-amplified luminol CL could be applied to selective detection of glucose in human plasma samples. Furthermore, such a uniformity-dependent CL enhancement effect could adapted to other redox CL systems-for example, the peroxynitrous acid (ONOOH) CL system.
在过去二十年中，纳米颗粒已被广泛应用于增强化学发光（CL）。纳米颗粒的形态对纳米颗粒放大的化学发光有重要影响。然而，纳米颗粒放大化学发光的研究主要集中在尺寸和形状效应上，尚未有人尝试探索纳米颗粒均匀性在纳米颗粒放大化学发光过程中的影响。在本研究中，我们以层状双氢氧化物（LDHs）作为模型材料，研究了鲁米诺-H₂O₂化学发光体系中的纳米颗粒均匀性。结果表明，LDHs的均匀性在化学发光放大中起关键作用。一种可能的机制是，具有高均匀性的LDHs拥有丰富的催化活性位点，这导致了高化学发光强度。同时，对H₂O₂检测的灵敏度提高了一个数量级（1.0 nM）。此外，均匀LDH放大的鲁米诺化学发光可用于人血浆样品中葡萄糖的选择性检测。此外，这种依赖于均匀性的化学发光增强效应可适用于其他氧化还原化学发光体系，例如过氧亚硝酸（ONOOH）化学发光体系。
