通过基因组编辑实现免疫调节等位基因的哺乳动物种间替换。
Mammalian interspecies substitution of immune modulatory alleles by genome editing.
作者信息
Lillico Simon G, Proudfoot Chris, King Tim J, Tan Wenfang, Zhang Lei, Mardjuki Rachel, Paschon David E, Rebar Edward J, Urnov Fyodor D, Mileham Alan J, McLaren David G, Whitelaw C Bruce A
机构信息
The Roslin Institute and R(D)SVS, Easter Bush Campus, University of Edinburgh, Edinburgh, EH25 9RG, UK.
Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, CA 94804, USA.
出版信息
Sci Rep. 2016 Feb 22;6:21645. doi: 10.1038/srep21645.
We describe a fundamentally novel feat of animal genetic engineering: the precise and efficient substitution of an agronomic haplotype into a domesticated species. Zinc finger nuclease in-embryo editing of the RELA locus generated live born domestic pigs with the warthog RELA orthologue, associated with resilience to African Swine Fever. The ability to efficiently achieve interspecies allele introgression in one generation opens unprecedented opportunities for agriculture and basic research.
我们描述了一项动物基因工程的全新壮举
将一种农艺单倍型精确且高效地替换到家养物种中。锌指核酸酶对RELA基因座进行胚胎内编辑，产生了携带疣猪RELA直系同源基因的活体家猪，该基因与对非洲猪瘟的抵抗力相关。能够在一代中高效实现种间等位基因渗入，为农业和基础研究带来了前所未有的机遇。
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