来自中华开口箭根茎的生物活性螺甾烷醇皂苷。

Bioactive spirostanol saponins from the rhizome of Tupistra chinensis.

作者信息

Xiang Limin, Wang Yihai, Yi Xiaomin, Zheng Guangji, He Xiangjiu

机构信息

School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China.

School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.

出版信息

Steroids. 2016 Apr;108:39-46. doi: 10.1016/j.steroids.2016.02.012. Epub 2016 Feb 18.

DOI:10.1016/j.steroids.2016.02.012

PMID:26898537

Abstract

Phytochemical investigations of the rhizome of Tupistra chinensis led to the isolation of six new spirostanol saponins, one new spirostanol, along with eight known spirostanols. Their chemical structures were elucidated on the basis of spectroscopic and chemical methods, including IR, NMR, MS, and GC analyses. The antiproliferative effects against five human cancer cell lines were assayed for all the isolated compounds. Compounds 8, 12 and 15 showed potent cytotoxic activities against K562 cells. The isolated compounds were evaluated for their inhibitory activities on nitric oxide (NO) production induced by lipopolysaccharide in a macrophage cell line RAW 264.7. Compounds 2 and 12 showed significant inhibition on NO production with IC50 values of 16.1±1.8 and 13.5±1.2 μM, respectively.

摘要

对开口箭根茎进行植物化学研究，从中分离出6个新的螺甾烷醇皂苷、1个新的螺甾烷醇以及8个已知的螺甾烷醇。通过红外光谱（IR）、核磁共振（NMR）、质谱（MS）和气相色谱（GC）分析等光谱和化学方法阐明了它们的化学结构。对所有分离得到的化合物测定了其对5种人类癌细胞系的抗增殖作用。化合物8、12和15对K562细胞显示出较强的细胞毒活性。评估了分离得到的化合物对巨噬细胞系RAW 264.7中脂多糖诱导的一氧化氮（NO）产生的抑制活性。化合物2和12对NO产生显示出显著抑制作用，IC50值分别为16.1±1.8和13.5±1.2 μM。