Keleher C A, Passmore S, Johnson A D
Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
Mol Cell Biol. 1989 Nov;9(11):5228-30. doi: 10.1128/mcb.9.11.5228-5230.1989.
To bring about repression of a family fo genes in Saccharomyces cerevisiae called the a-specific genes, two transcriptional regulatory proteins, alpha 2 and GRM (general regulator of matin type), bind cooperatively to an operator found upstream of each a-specific gene. To date, GRM has been defined only biochemically. In this communication we show that the product of a single yeast gene (MCM1) is sufficient to bind cooperatively with alpha 2 to the operator. We also show that antiserum raised against the MCM1 gene product recognizes GRM from yeast cells. These results, in combination with previous observations, provide strong evidence that MCM1 encodes the GRM activity.
为了抑制酿酒酵母中一组被称为a特异性基因的基因家族，两种转录调节蛋白α2和GRM（交配型通用调节因子）协同结合到每个a特异性基因上游的一个操纵子上上。到每个a特异性基因上游的一个操纵子。迄今为止，GRM仅在生化水平上得到定义。在本通讯中，我们表明单个酵母基因（MCM1）的产物足以与α2协同结合到该操纵子。我们还表明，针对MCM1基因产物产生的抗血清能识别酵母细胞中的GRM。这些结果与先前的观察结果相结合，提供了强有力的证据，表明MCM1编码GRM活性。
