Bohacsova Monika, Mediannikov Oleg, Kazimirova Maria, Raoult Didier, Sekeyova Zuzana
Department of Rickettsiology, Institute of Virology, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia.
Unite de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE) IRD 198, CNRS 7278, INSERM 1095, Institut Hospitalo-Universitaire (IHU) Mediterranee-Infection, Aix-Marseille University, Marseille, France.
PLoS One. 2016 Feb 22;11(2):e0149950. doi: 10.1371/journal.pone.0149950. eCollection 2016.
Arsenophonus nasoniae, a male-killing endosymbiont of chalcid wasps, was recently detected in several hard tick species. Following the hypothesis that its presence in ticks may not be linked to the direct occurrence of bacteria in tick's organs, we identified A. nasoniae in wasps emerging from parasitised nymphs. We confirmed that 28.1% of Ixodiphagus hookeri wasps parasitizing Ixodes ricinus ticks were infected by A. nasoniae. Moreover, in examined I. ricinus nymphs, A. nasoniae was detected only in those, which were parasitized by the wasp. However, in part of the adult wasps as well as in some ticks that contained wasp's DNA, we did not confirm A. nasoniae. We also found, that in spite of reported male-killing, some newly emerged adult wasp males were also infected by A. nasoniae. Additionally, we amplified the DNA of Rickettsia helvetica and Rickettsia monacensis (known to be Ixodes ricinus-associated bacteria) in adult parasitoid wasps. This may be related either with the digested bacterial DNA in wasp body lumen or with a role of wasps in circulation of rickettsiae among tick vectors.
纳氏阿氏菌是一种寄生于小蜂科昆虫的杀雄内共生菌，最近在几种硬蜱物种中被发现。基于其在蜱虫体内的存在可能与蜱虫器官中细菌的直接存在无关这一假设，我们在被寄生若虫羽化出的小蜂中鉴定出了纳氏阿氏菌。我们证实，寄生在蓖麻硬蜱上的钩形嗜蜱小蜂中有28.1%被纳氏阿氏菌感染。此外，在所检查的蓖麻硬蜱若虫中，仅在被该小蜂寄生的若虫中检测到了纳氏阿氏菌。然而，在部分成年小蜂以及一些含有小蜂DNA的蜱虫中，我们并未证实存在纳氏阿氏菌。我们还发现，尽管有报道称存在杀雄现象，但一些新羽化的成年雄蜂也被纳氏阿氏菌感染。此外，我们在成年寄生蜂中扩增出了瑞士立克次体和蒙氏立克次体（已知与蓖麻硬蜱相关的细菌）的DNA。这可能与蜂体内腔中被消化的细菌DNA有关，或者与黄蜂在立克次体在蜱虫媒介间传播中的作用有关。
