Zhang Yang, Wang Faxuan, Zhou Dingzi, Ren Xiaohui, Zhou Dinglun, Gao Xiaosi, Lan Yajia, Zhang Qin, Xie Xiaoqi
Department of Occupational and Environmental Medicine, West China School of Public Health, Sichuan University, China.
Ind Health. 2016 Aug 5;54(4):361-9. doi: 10.2486/indhealth.2015-0170. Epub 2016 Feb 20.
Background To identify differentially expressed miRNAs profiles in bronchoalveolar lavage fluid (BALF) from patients with silicosis and consider the potential contribution of miRNAs to silicosis.Methods miRNAs expression profiling were performed in the cell fraction of BALF samples obtained from 9 subjects (3 silicosis observation subjects, 3 stage I and stage II silicosis patients, respectively). The differential expression of two selected miRNAs hsa-miR-181c-5p and hsa-miR-29a-3p were confirmed by RT-qPCR. Furthermore, miRNAs Gene Ontology Enrichment categories and target mRNAs were determined based on miRWalk.Results We found 110 dysregulated miRNAs in silicosis samples, most of which showed a down-regulation trend. Microarray results were confirmed by RT-qPCR. With the observation group samples set as standards, stage I samples showed 123 differentially expressed miRNAs, and stage II 46. 23 miRNAs were dysregulated in both stages. Finally, functional enrichment analysis indicated that these miRNAs played an important role in various biological processes, including ECM-receptor interaction and endocytosis.Conclusions This is the first time to acquire the BALF-derived microRNAs expression profiling targeting to human silicosis. These results contribute to unravelling miRNAs involved in the pathogenesis of silicosis, and provide new tools of potential use of as biomarkers for diagnosis and/or therapeutic purposes.
识别矽肺患者支气管肺泡灌洗液（BALF）中差异表达的miRNA谱，并探讨miRNA对矽肺的潜在作用。方法：对9名受试者（3名矽肺观察对象、3名I期和II期矽肺患者）的BALF样本细胞成分进行miRNA表达谱分析。通过RT-qPCR确认了两个选定的miRNA即hsa-miR-181c-5p和hsa-miR-29a-3p的差异表达。此外，基于miRWalk确定了miRNA基因本体富集类别和靶mRNA。结果：我们在矽肺样本中发现了110个失调的miRNA，其中大多数呈下调趋势。微阵列结果通过RT-qPCR得到证实。以观察组样本为标准，I期样本显示123个差异表达的miRNA，II期样本显示46个。两个阶段共有23个miRNA失调。最后，功能富集分析表明这些miRNA在各种生物学过程中发挥重要作用，包括细胞外基质-受体相互作用和内吞作用。结论：这是首次获得针对人类矽肺的BALF来源的microRNA表达谱。这些结果有助于揭示参与矽肺发病机制的miRNA，并为诊断和/或治疗目的提供潜在的生物标志物新工具。
