Brock Matthias, Rechsteiner Thomas, Kohler Malcolm, Franzen Daniel, Huber Lars C
Division of Pulmonology, University Hospital Zurich, Zurich, Switzerland.
Lung. 2015 Jun;193(3):381-5. doi: 10.1007/s00408-015-9719-5. Epub 2015 Mar 21.
Levels of microRNAs (miRNAs) are increasingly assessed in biological fluids, for example, in samples obtained by bronchoalveolar lavage (BAL). "Post-collection kinetics" of miRNA expression levels, however, have not been investigated to date. In these experiments, we analyzed the dynamic expression profile of 5 different miRNAs (miR-17, miR-19b, miR-20b, miR-125a, and miR-223-3p) in BAL within the first 24 h following collection by routine bronchoscopy. miRNAs were quantified 0, 1, 4, 8, and 24 h after collection in samples that were kept at 4 °C or at room temperature. The expression of all five miRNAs was found to remain stable between the first 8 h after collection. 24 h after collection miRNAs faced substantial alterations in their expression profile. These data emphasize that BAL samples intended for further miRNA analysis can be handled at room temperature within the first 8 h after bronchoscopy.
目前越来越多地在生物体液中评估微小RNA（miRNA）的水平，例如通过支气管肺泡灌洗（BAL）获得的样本。然而，迄今为止尚未对miRNA表达水平的“采集后动力学”进行研究。在这些实验中，我们分析了通过常规支气管镜检查采集后的前24小时内，BAL中5种不同miRNA（miR-17、miR-19b、miR-20b、miR-125a和miR-223-3p）的动态表达谱。在采集后0、1、4、8和24小时，对保存在4°C或室温下的样本中的miRNA进行定量。发现所有五种miRNA的表达在采集后的前8小时内保持稳定。采集后24小时，miRNA的表达谱发生了显著变化。这些数据强调，用于进一步miRNA分析的BAL样本在支气管镜检查后的前8小时内可以在室温下处理。
