Yu Shaolan, Yao Peng, Liu Jiwen, Zhao Bin, Zhang Guiling, Zhao Meixun, Yu Zhigang, Zhang Xiao-Hua
Laboratory of Marine Microbiology, College of Marine Life Sciences, Ocean University of China Qingdao, China.
Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of ChinaQingdao, China; Qingdao Collaborative Innovation Center of Marine Science and Technology, Ocean University of ChinaQingdao, China; Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and TechnologyQingdao, China.
Front Microbiol. 2016 Feb 12;7:137. doi: 10.3389/fmicb.2016.00137. eCollection 2016.
The eastern China marginal seas (ECMS) are prominent examples of river-dominated ocean margins, whose most characteristic feature is the existence of isolated mud patches on sandy sediments. Ammonia-oxidizing prokaryotes play a crucial role in the nitrogen cycles of many marine environments, including marginal seas. However, few studies have attempted to address the distribution patterns of ammonia-oxidizing prokaryotes in mud deposits of these seas. The horizontal and vertical community composition and abundance of ammonia-oxidizing archaea (AOA) and bacteria (AOB) were investigated in mud deposits of the South Yellow Sea (SYS) and the East China Sea (ECS) by using amoA clone libraries and quantitative PCR. The diversity of AOB was comparable or higher in the mud zone of SYS and lower in ECS when compared with AOA. Vertically, surface sediments had generally higher diversity of AOA and AOB than middle and bottom layers. Diversity of AOA and AOB showed significant correlation with latitude. Nitrosopumilus and Nitrosospira lineages dominated AOA and AOB communities, respectively. Both AOA and AOB assemblages exhibited greater variations across different sites than those among various depths at one site. The abundance of bacterial amoA was generally higher than that of archaeal amoA, and both of them decreased with depth. Niche differentiation, which was affected by dissolved oxygen, salinity, ammonia, and silicate (SiO[Formula: see text]), was observed between AOA and AOB and among different groups of them. The spatial distribution of AOA and AOB was significantly correlated with δ(15)NTN and SiO[Formula: see text], and nitrate and δ(13)C, respectively. Both archaeal and bacterial amoA abundance correlated strongly with SiO[Formula: see text]. This study improves our understanding of spatial distribution of AOA and AOB in ecosystems featuring oceanic mud deposits.
中国东部边缘海是典型的河流主导型海洋边缘，其最显著的特征是在沙质沉积物上存在孤立的泥斑。氨氧化原核生物在包括边缘海在内的许多海洋环境的氮循环中起着关键作用。然而，很少有研究试图探讨这些海域泥质沉积物中氨氧化原核生物的分布模式。本研究利用amoA克隆文库和定量PCR技术，对南黄海（SYS）和东海（ECS）泥质沉积物中氨氧化古菌（AOA）和细菌（AOB）的水平和垂直群落组成及丰度进行了调查。与AOA相比，SYS泥质区AOB的多样性相当或更高，而ECS中AOB的多样性较低。垂直方向上，表层沉积物中AOA和AOB的多样性通常高于中层和底层。AOA和AOB的多样性与纬度呈显著相关。Nitrosopumilus和Nitrosospira谱系分别主导着AOA和AOB群落。与一个站点不同深度之间的差异相比，AOA和AOB组合在不同站点之间的变化更大。细菌amoA的丰度通常高于古菌amoA，且二者均随深度降低。在AOA和AOB之间以及它们的不同群体之间观察到生态位分化，这受到溶解氧、盐度、氨和硅酸盐（SiO₄）的影响。AOA和AOB的空间分布分别与δ(¹⁵)NTN和SiO₄、硝酸盐和δ(¹³)C显著相关。古菌和细菌amoA的丰度均与SiO₄密切相关。本研究增进了我们对以海洋泥质沉积物为特征的生态系统中AOA和AOB空间分布的理解。
