Koyama Taro, Kobayashi Kazuhiro, Hama Takanori, Murakami Kasumi, Ogawa Rei
Men's Health Clinic Tokyo, Tokyo, Japan; Department of Plastic, Reconstructive and Aesthetic Surgery, Nippon Medical School, Tokyo, Japan.
Men's Health Clinic Tokyo, Tokyo, Japan.
Eplasty. 2016 Jan 25;16:e8. eCollection 2016.
In this study, we evaluated the effect of scalp massage on hair in Japanese males and the effect of stretching forces on human dermal papilla cells in vitro.
Nine healthy men received 4 minutes of standardized scalp massage per day for 24 weeks using a scalp massage device. Total hair number, hair thickness, and hair growth rate were evaluated. The mechanical effect of scalp massage on subcutaneous tissue was analyzed using a finite element method. To evaluate the effect of mechanical forces, human dermal papilla cells were cultured using a 72-hour stretching cycle. Gene expression change was analyzed using DNA microarray analyses. In addition, expression of hair cycle-related genes including IL6, NOGGIN, BMP4, and SMAD4 were evaluated using real-time reverse transcription-polymerase chain reaction.
Standardized scalp massage resulted in increased hair thickness 24 weeks after initiation of massage (0.085 ± 0.003 mm vs 0.092 ± 0.001 mm). Finite element method showed that scalp massage caused z-direction displacement and von Mises stress on subcutaneous tissue. In vitro, DNA microarray showed gene expression change significantly compared with nonstretching human dermal papilla cells. A total of 2655 genes were upregulated and 2823 genes were downregulated. Real-time reverse transcription-polymerase chain reaction demonstrated increased expression of hair cycle-related genes such as NOGGIN, BMP4, SMAD4, and IL6ST and decrease in hair loss-related genes such as IL6.
Stretching forces result in changes in gene expression in human dermal papilla cells. Standardized scalp massage is a way to transmit mechanical stress to human dermal papilla cells in subcutaneous tissue. Hair thickness was shown to increase with standardized scalp massage.
在本研究中，我们评估了头皮按摩对日本男性头发的影响以及拉伸力对体外人毛乳头细胞的影响。
9名健康男性使用头皮按摩装置，每天接受4分钟的标准化头皮按摩，持续24周。评估头发总数、头发厚度和头发生长速率。使用有限元方法分析头皮按摩对皮下组织的力学效应。为评估机械力的作用，采用72小时拉伸周期培养人毛乳头细胞。使用DNA微阵列分析技术分析基因表达变化。此外，使用实时逆转录-聚合酶链反应评估包括IL6、NOGGIN、BMP4和SMAD4在内的毛发周期相关基因的表达。
标准化头皮按摩在按摩开始24周后导致头发厚度增加（0.085±0.003毫米对0.092±0.001毫米）。有限元方法显示头皮按摩导致皮下组织在z方向的位移和冯·米塞斯应力。在体外，与未拉伸的人毛乳头细胞相比，DNA微阵列显示基因表达有显著变化。共有2655个基因上调，2823个基因下调。实时逆转录-聚合酶链反应表明毛发周期相关基因如NOGGIN、BMP4、SMAD4和IL6ST的表达增加，而脱发相关基因如IL6的表达减少。
拉伸力导致人毛乳头细胞基因表达发生变化。标准化头皮按摩是将机械应力传递至皮下组织中人毛乳头细胞的一种方式。标准化头皮按摩可使头发厚度增加。
