Samarghandian Saeed, Farkhondeh Tahereh, Samini Fariborz, Borji Abasalt
Department of Basic Medical Sciences, Neyshabur University of Medical Sciences, Neyshabur 14139-93186, Iran.
Department of Immunogenetics, BuAli Research Institute, Mashhad University of Medical Sciences, Mashhad 9196773117, Iran.
Biochem Res Int. 2016;2016:2645237. doi: 10.1155/2016/2645237. Epub 2016 Jan 19.
Restraint stress may be associated with elevated free radicals, and thus, chronic exposure to oxidative stress may cause tissue damage. Several studies have reported that carvacrol (CAR) has a protective effect against oxidative stress. The present study was designed to investigate the protective effects of CAR on restraint stress induced oxidative stress damage in the brain, liver, and kidney. For chronic restraint stress, rats were kept in the restrainers for 6 h every day, for 21 consecutive days. The animals received systemic administrations of CAR daily for 21 days. To evaluate the changes of the oxidative stress parameters following restraint stress, the levels of malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), and catalase (CAT) activities were measured in the brain, liver, and kidney. In the stressed animals that received vehicle, the MDA level was significantly higher (P < 0.001) and the levels of GSH and antioxidant enzymes were significantly lower than the nonstressed animals (P < 0.001). CAR ameliorated the changes in the stressed animals as compared with the control group (P < 0.001). This study indicates that CAR can prevent restraint stress induced oxidative damage.
束缚应激可能与自由基升高有关，因此，长期暴露于氧化应激可能会导致组织损伤。多项研究报告称香芹酚（CAR）对氧化应激具有保护作用。本研究旨在探讨香芹酚对束缚应激诱导的脑、肝和肾氧化应激损伤的保护作用。对于慢性束缚应激，大鼠每天在束缚器中放置6小时，连续21天。动物每天接受香芹酚全身给药，持续21天。为了评估束缚应激后氧化应激参数的变化，在脑、肝和肾中测量丙二醛（MDA）、还原型谷胱甘肽（GSH）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）、谷胱甘肽还原酶（GR）和过氧化氢酶（CAT）活性水平。在接受赋形剂的应激动物中，MDA水平显著更高（P < 0.001），GSH和抗氧化酶水平显著低于非应激动物（P < 0.001）。与对照组相比，香芹酚改善了应激动物的变化（P < 0.001）。本研究表明，香芹酚可以预防束缚应激诱导的氧化损伤。
