Cyganek Anna, Wyczalkowska-Tomasik Aleksandra, Jarmuzek Patrycja, Grzechocinska Barbara, Jabiry-Zieniewicz Zoulikha, Paczek Leszek, Wielgos Miroslaw
1st Department of Obstetrics and Gynecology, Medical University of Warsaw, Starynkiewicza 1/3, 02-015 Warsaw, Poland.
Department of Immunology, Transplantology and Internal Diseases, Transplantation Institute, Medical University of Warsaw, Warsaw, Poland.
Biomed Res Int. 2016;2016:7065821. doi: 10.1155/2016/7065821. Epub 2016 Jan 20.
Objectives. The aim of the study was to evaluate the activity of cathepsin B, collagenases, trypsin, and plasmin and concentration of cystatin C in serum of healthy pregnant women in peripartum period. Study Design. The study group included 45 women in uncomplicated pregnancies. Blood samples were collected in four time points. Enzyme activity was measured by spectrofluorometric method. The level of cystatin C was measured using immunonephelometric method. Results. Mean activity of cathepsin B and the level of serum cystatin C were significantly higher in the study group. Collagenase activity was significantly lower in the study group than the control group. No differences in collagenase, plasmin, and trypsin activity on each day of the peripartum period were found. Conclusion. High activity of cathepsin B and increased level of cystatin C are typical for women in late pregnancy. Those levels significantly decrease after delivery which can be associated with potential role of those markers in placental separation. The insignificant changes of cystatin C level in the peripartum period seem to exclude the possibility of using cystatin C as a marker for renal insufficiency in the peripartum period but additional research is necessary to investigate the matter further.
目的。本研究旨在评估围产期健康孕妇血清中组织蛋白酶B、胶原酶、胰蛋白酶和纤溶酶的活性以及胱抑素C的浓度。研究设计。研究组包括45例无并发症妊娠的妇女。在四个时间点采集血样。酶活性通过荧光分光光度法测量。胱抑素C水平采用免疫比浊法测量。结果。研究组中组织蛋白酶B的平均活性和血清胱抑素C水平显著更高。研究组的胶原酶活性显著低于对照组。在围产期的每一天，胶原酶、纤溶酶和胰蛋白酶活性均未发现差异。结论。组织蛋白酶B的高活性和胱抑素C水平升高是晚期妊娠女性的典型特征。分娩后这些水平显著降低，这可能与这些标志物在胎盘剥离中的潜在作用有关。围产期胱抑素C水平的微小变化似乎排除了将胱抑素C用作围产期肾功能不全标志物的可能性，但需要进一步的研究来进一步调查此事。
