口胃癌患者念珠菌属的分离与鉴定：对抗真菌药物的敏感性、体外毒力特性及免疫反应表型
Isolation and identification of Candida species in patients with orogastric cancer: susceptibility to antifungal drugs, attributes of virulence in vitro and immune response phenotype.
作者信息
de Sousa Lourimar Viana Nascimento F, Santos Vera Lúcia, de Souza Monteiro Andrea, Dias-Souza Marcus Vinicíus, Marques Sirlei Garcia, de Faria Elaine Speziali, Assunção Elaine Alves de Oliveira, Dos Santos Simone Gonçalves, Zonis Juan Moises, de Alvarenga Daniel Gomes, de Holanda Rodrigo Assunção, de Sousa Jaqueline Gontijo, Dos Santos Kênia Valéria, Stoianoff Maria Aparecida de Resende
机构信息
Laboratory of Mycology, Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
Laboratory of Microbiology, Vale do Rio Doce University, Governador Valadares, MG, Brazil.
出版信息
BMC Infect Dis. 2016 Feb 23;16:86. doi: 10.1186/s12879-016-1431-4.
BACKGROUND
Because of the inherent immunosuppression of cancer patients opportunistic infections by Candida spp, occur frequently. This study aimed to identify Candida species in the oral mucosa of 59 patients with orogastric cancer (OGC) and to analyze the immunological phenotype of these patients.
METHODS
The yeasts were identified by MALDI-TOF mass spectrometry (MS). For all isolates, we performed phospholipases and proteinases assays, in vitro adherence to buccal epithelial cells (BEC), minimum inhibitory concentration of antifungal drugs and determined the cytokine profile by Cytometric Bead Array flow citometry assay.
RESULTS
C. albicans was the most prevalent species in OGC patients (51.6 %) and control group (66.7 %). Candida spp. strains isolated from OGC patients exhibited better adherence to BEC (p = 0.05) than did the control group. Phospholipases production by Candida strains from OGC patients was lower (51.6 %) than in the control group (61.9 %). Proteinases were detected in 41.9 % and 4.8 % of the yeasts from OGC patients and control group, respectively. Significant differences were found in the serum of OGC patients compared to the control group for IL-2, IL-10, TNF-α, IFN-γ and IL-17.
CONCLUSIONS
The results of this work suggest increased virulence of yeasts isolated from OGC patients and, that this may interfere with the immune phenotype.
背景
由于癌症患者存在内在的免疫抑制，念珠菌属引起的机会性感染频繁发生。本研究旨在鉴定59例口胃癌（OGC）患者口腔黏膜中的念珠菌种类，并分析这些患者的免疫表型。
方法
通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）鉴定酵母。对于所有分离株，我们进行了磷脂酶和蛋白酶检测、体外对颊上皮细胞（BEC）的黏附、抗真菌药物的最低抑菌浓度测定，并通过细胞计数微珠阵列流式细胞术检测细胞因子谱。
结果
白色念珠菌是OGC患者（51.6%）和对照组（66.7%）中最常见的菌种。从OGC患者分离的念珠菌属菌株对BEC的黏附性（p = 0.05）高于对照组。OGC患者分离的念珠菌菌株产生的磷脂酶（51.6%）低于对照组（61.9%）。分别在41.9%的OGC患者酵母和4.8%的对照组酵母中检测到蛋白酶。与对照组相比，OGC患者血清中的IL-2、IL-10、TNF-α、IFN-γ和IL-17存在显著差异。
结论
这项工作的结果表明，从OGC患者分离的酵母毒力增加，这可能会干扰免疫表型。
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