Wijesinghe Printha, Shankar S K, Chickabasaviah Yasha T, Gorrie Catherine, Amaratunga Dhammika, Hulathduwa Sanjayah, Kumara K Sunil, Samarasinghe Kamani, Suh Yoo Hun, Steinbusch H W, De Silva K Ranil D
Genetic Diagnostic and Research Laboratory, Department of Anatomy, Faculty of Medical Sciences, University of Sri Jayewardenepura, Nugegoda, Postal Code: 10250, Sri Lanka.
Curr Alzheimer Res. 2016;13(3):268-80. doi: 10.2174/156720501303160217121210.
Within South Asia, Sri Lanka represents fastest aging with 13% of the population was aged over 60's in 2011, whereas in India it was 8%. Majority of the Sri Lankan population based genetic studies have confirmed their origin on Indian mainland. As there were inadequate data on aging cytoskeletal pathologies of these two nations with their close genetic affiliations, we performed a comparison on their elderly. Autopsy brain samples of 50 individuals from Colombo, Sri Lanka (mean age 72.1 yrs ± 7.8, mean ± S.D.) and 42 individuals from Bangalore, India (mean age 65.9 yrs ± 9.3) were screened for neurodegenerative pathologies using immunohistochemical techniques. A total of 79 cases with incomplete clinical history (Colombo- 47 and Bangalore- 32) were subjected to statistical analysis and 13 cases, clinically diagnosed with dementia and/or Parkinsonism disorders were excluded. As per National Institute on Aging- Alzheimer's Association guidelines, between Colombo and Bangalore samples, Alzheimer's disease neuropathologic change for intermediate/ high level was 4.25% vs. 3.12% and low level was 19.15% vs. 15.62% respectively. Pathologies associated with Parkinsonism including brainstem predominant Lewy bodies- 6.4% and probable progressive supra nuclear palsy- 2.13% were found solely in Colombo samples. Alzheimer related pathologies were not different among elders, however, in Colombo males, neurofibrillary tangle grade was significantly higher in the region of hippocampus (odds ratio = 1.46, 95% confidence interval = 0.07-0.7) and at risk in midbrain substantia nigra (p = 0.075). Other age-related pathologies including spongiform changes (p < 0.05) and hippocampus cell loss in dentate gyrus region (p < 0.05) were also identified prominently in Colombo samples. Taken together, aging cytoskeletal pathologies are comparatively higher in elderly Sri Lankans and this might be due to their genetic, dietary and/ or environmental variations.
在南亚地区，斯里兰卡老龄化速度最快，2011年60岁以上人口占比达13%，而印度这一比例为8%。多数基于斯里兰卡人口的基因研究证实他们起源于印度大陆。鉴于这两个有着密切基因关联的国家关于衰老细胞骨架病理学的数据不足，我们对其老年人进行了比较。使用免疫组织化学技术，对来自斯里兰卡科伦坡的50例个体（平均年龄72.1岁±7.8，均值±标准差）和来自印度班加罗尔的42例个体（平均年龄65.9岁±9.3）的尸检脑样本进行神经退行性病变筛查。对总共79例临床病史不完整的病例（科伦坡47例，班加罗尔32例）进行统计分析，并排除13例临床诊断为痴呆和/或帕金森症的病例。根据美国国立衰老研究所 - 阿尔茨海默病协会的指南，在科伦坡和班加罗尔的样本中，阿尔茨海默病中级/高级神经病理变化分别为4.25%对3.12%，低级变化分别为19.15%对15.62%。仅在科伦坡样本中发现与帕金森症相关的病变，包括脑干为主的路易小体占6.4%以及可能的进行性核上性麻痹占2.13%。阿尔茨海默相关病变在老年人中并无差异，然而，在科伦坡男性中，海马区神经原纤维缠结等级显著更高（优势比 = 1.46，95%置信区间 = 0.07 - 0.7），且中脑黑质有风险（p = 0.075）。其他与年龄相关的病变，包括海绵状变化（p < 0.05）和齿状回区域海马细胞丢失（p < 0.05），在科伦坡样本中也显著存在。总体而言，斯里兰卡老年人的衰老细胞骨架病理学情况相对较高，这可能归因于他们的基因、饮食和/或环境差异。
