Durand M, Sermondade N, Herbemont C, Benard J, Gronier H, Boujenah J, Cédrin-Durnerin I, Poncelet C, Grynberg M, Sifer C
Service d'histologie-embryologie-cytogénétique-CECOS, centre hospitalo-universitaire Jean-Verdier, Assistance publique-Hôpitaux de Paris, avenue du 14-Juillet, 93140 Bondy, France.
Service d'histologie-embryologie-cytogénétique-CECOS, centre hospitalo-universitaire Jean-Verdier, Assistance publique-Hôpitaux de Paris, avenue du 14-Juillet, 93140 Bondy, France.
Gynecol Obstet Fertil. 2016 Mar;44(3):163-7. doi: 10.1016/j.gyobfe.2016.01.005. Epub 2016 Feb 18.
The aim of this study was to compare embryo development cultured in two single-step media commercially available: Fert/Sage One Step® (Origio) and Continuous Single Culture® (CSC) (Irvine Scientific).
A prospective auto-controlled study of sibling oocytes from women undergoing conventional in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) was performed in our center from February to June 2015. After fertilization, for every patient, half of oocytes were cultured in the single-step Fert/Sage One Step® (serie SAGE) and the other half in the single-step CSC®(serie CSC). Fertilization and embryo morphology rates were assessed by day 1 to day 5-6 if needed. Embryo presenting<20% of fragmentation and 4 cells at day 2, 8 cells at day 3 were qualified as "top quality". Embryo with<20% of fragmentation and 3-5 cells at day 2, 6-10 cells at day 3 were qualified as "good quality". Blastocyst B3, B4, B5 with A or B inner cell mass and A or B trophectoderm were qualified as "good quality". Transferred or frozen embryos were qualified as useful embryos.
Sixty-two attempts of IVF and 133 of ICSI were analyzed, corresponding to 2059 inseminated or micro-injected oocytes. Fertilization rate were not different between the 2 series, respectively SAGE vs CSC (IVF: 73.4% vs 68.3% [P=0.49]; ICSI: 58.9% vs 63.8% [P=0.12]). No difference was found for embryo morphology, respectively SAGE vs CSC, at day 2 (top quality embryo at day 2 IVF: 34.4% vs 33% [P=0.98]; ICSI: 42.4% vs 44.9% [P=0.37]; and good quality embryo at day 2 IVF: 44% vs 50.2% [P=0.07]; ICSI: 64% vs 71% [P=0.35]); no difference at day 3 (top quality embryo at day 3 IVF: 19.4% vs 21.3% [P=0.61]; ICSI: 28.7% vs 27.4% [P=0.54]; and good quality embryo at day 3 IVF: 40.4% vs 50.2% [P=0.91]; ICSI: 51% vs 47.6% [P=0.47]). Blastocyst development rate were not different, respectively SAGE vs CSC (IVF: 39.9% vs 41.5% [P=0.63] with 42.9% vs 42.2% of good quality blastocyst [P=0.70]; ICSI: 41.1% vs 37.8% [P=0.18] with 32.9% vs 40.8% of good quality blastocyst [P=0.13]). No difference was found in the useful embryo rate in the 2 series SAGE vs CSC (IVF: 52.8% vs 55.2% [P=0.83]; ICSI: 62.4% vs 61.7% [P=0.70]).
Embryo development and rate of useful embryos, transferred or frozen, were not different according to the embryo culture in single-step media Fert/Sage One Step® vs single-step Continuous Single Culture®.
本研究旨在比较两种市售单步培养基中培养的胚胎发育情况：Fert/Sage One Step®（奥瑞金公司）和连续单一培养（CSC）（欧文科学公司）。
2015年2月至6月在我们中心对接受常规体外受精（IVF）或卵胞浆内单精子注射（ICSI）的女性的同胞卵母细胞进行了前瞻性自身对照研究。受精后，对每位患者，一半卵母细胞在单步Fert/Sage One Step®（SAGE组）中培养，另一半在单步CSC®（CSC组）中培养。必要时在第1天至第5 - 6天评估受精率和胚胎形态。第2天碎片率<20%且有4个细胞、第3天有8个细胞的胚胎被判定为“优质”。第2天碎片率<20%且有3 - 5个细胞、第3天有6 - 10个细胞的胚胎被判定为“良好”。内细胞团为A或B级、滋养外胚层为A或B级的囊胚B3、B4、B5被判定为“良好”。移植或冷冻的胚胎被判定为可用胚胎。
分析了62次IVF尝试和133次ICSI尝试，对应2059个受精或显微注射的卵母细胞。两组的受精率无差异，分别为SAGE组与CSC组（IVF：73.4%对68.3% [P = 0.49]；ICSI：58.9%对63.8% [P = 0.12]）。在第2天，两组胚胎形态无差异，分别为SAGE组与CSC组（IVF第2天优质胚胎：34.4%对33% [P = 0.98]；ICSI：42.4%对44.9% [P = 0.37]；IVF第2天良好胚胎：44%对50.2% [P = 0.07]；ICSI：64%对71% [P = 0.35]）；在第3天也无差异（IVF第3天优质胚胎：19.4%对21.3% [P = 0.61]；ICSI：28.7%对27.4% [P = 0.54]；IVF第3天良好胚胎：40.4%对50.2% [P = 0.91]；ICSI：51%对47.6% [P = 0.47]）。囊胚发育率无差异，分别为SAGE组与CSC组（IVF：39.9%对41.5% [P = 0.63]，优质囊胚率为42.9%对42.2% [P = 0.70]；ICSI：41.1%对37.8% [P = 0.18]，优质囊胚率为32.9%对40.8% [P = 0.13]）。两组可用胚胎率无差异，分别为SAGE组与CSC组（IVF：52.8%对55.2% [P = 0.83]；ICSI：62.4%对61.7% [P = 0.70]）。
根据在单步培养基Fert/Sage One Step®与单步连续单一培养CSC®中培养胚胎的情况，胚胎发育以及移植或冷冻的可用胚胎率无差异。
