一项自动控制的前瞻性比较研究，比较两种胚胎培养液（G III 系列与 ISM）在 IVF 和 ICSI 治疗中的效果。
An auto-controlled prospective comparison of two embryos culture media (G III series versus ISM) for IVF and ICSI treatments.
机构信息
Service d'Histologie-Embryologie-Cytogénétique, Laboratoire de Biologie de la Reproduction, Centre Hospitalier Universitaire Jean Verdier, Assistance Publique-Hôpitaux de Paris, 93143 Bondy, France.
出版信息
J Assist Reprod Genet. 2009 Nov-Dec;26(11-12):575-81. doi: 10.1007/s10815-009-9357-z. Epub 2009 Nov 11.
PURPOSE
To compare the effects of 2 different media on embryo morphology and development at days 2/3.
METHOD
Six hundred seventy-six attempts from 512 couples were included in this prospective auto-controlled study. Sibling oocytes of all couples undergoing an IVF (n = 286) or ICSI (n = 390) attempt were randomly assigned to either GIII series (Vitrolife) or ISM (Medicult) media. Primary end points were fertilization and embryo morphology rates.
RESULTS
Fertilization rates in GIII series and ISM (IVF: 59.9 vs 62.0% and ICSI: 65.7 vs 66.8%) respectively were not different. GIII series showed an increase, compared to ISM, of early cleavage rate, (IVF: 25.8 vs 16.2% (p = 0.005); ICSI: 40.8 vs 25.5% (p < 0.0001), and good embryo morphology rate at day 2 [IVF: 64.6 vs 57.3% (p = 0.01); ICSI: 74.2 vs 69.4 (p = 0.03)] and at day 3 [IVF: 57.5 vs 49.0% (p = 0.02); ICSI: 67.2 vs 61.6% (p = 0.01)].
CONCLUSIONS
Embryo morphology at days 2/3 was significantly enhanced when the embryos were cultured in GIII series.
目的
比较两种不同培养基对第 2/3 天胚胎形态和发育的影响。
方法
本前瞻性自身对照研究纳入了 512 对夫妇的 676 次尝试。所有接受 IVF（n=286）或 ICSI（n=390）尝试的夫妇的同胞卵母细胞被随机分配到 GIII 系列（Vitrolife）或 ISM（Medicult）培养基中。主要终点是受精率和胚胎形态率。
结果
GIII 系列和 ISM 的受精率分别为（IVF：59.9% vs 62.0%和 ICSI：65.7% vs 66.8%），无差异。与 ISM 相比，GIII 系列显示早期分裂率增加，（IVF：25.8% vs 16.2%（p=0.005）；ICSI：40.8% vs 25.5%（p<0.0001），并且第 2 天的优质胚胎形态率更高[IVF：64.6% vs 57.3%（p=0.01）；ICSI：74.2% vs 69.4%（p=0.03）]和第 3 天[IVF：57.5% vs 49.0%（p=0.02）；ICSI：67.2% vs 61.6%（p=0.01）]。
结论
当胚胎在 GIII 系列中培养时，第 2/3 天的胚胎形态显著增强。
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