Service d'Histologie-Embryologie-Cytogénétique, Laboratoire de Biologie de la Reproduction, Centre Hospitalier Universitaire Jean Verdier, Assistance Publique-Hôpitaux de Paris, 93143 Bondy, France.
J Assist Reprod Genet. 2009 Nov-Dec;26(11-12):575-81. doi: 10.1007/s10815-009-9357-z. Epub 2009 Nov 11.
To compare the effects of 2 different media on embryo morphology and development at days 2/3.
Six hundred seventy-six attempts from 512 couples were included in this prospective auto-controlled study. Sibling oocytes of all couples undergoing an IVF (n = 286) or ICSI (n = 390) attempt were randomly assigned to either GIII series (Vitrolife) or ISM (Medicult) media. Primary end points were fertilization and embryo morphology rates.
Fertilization rates in GIII series and ISM (IVF: 59.9 vs 62.0% and ICSI: 65.7 vs 66.8%) respectively were not different. GIII series showed an increase, compared to ISM, of early cleavage rate, (IVF: 25.8 vs 16.2% (p = 0.005); ICSI: 40.8 vs 25.5% (p < 0.0001), and good embryo morphology rate at day 2 [IVF: 64.6 vs 57.3% (p = 0.01); ICSI: 74.2 vs 69.4 (p = 0.03)] and at day 3 [IVF: 57.5 vs 49.0% (p = 0.02); ICSI: 67.2 vs 61.6% (p = 0.01)].
Embryo morphology at days 2/3 was significantly enhanced when the embryos were cultured in GIII series.
比较两种不同培养基对第 2/3 天胚胎形态和发育的影响。
本前瞻性自身对照研究纳入了 512 对夫妇的 676 次尝试。所有接受 IVF（n=286）或 ICSI（n=390）尝试的夫妇的同胞卵母细胞被随机分配到 GIII 系列（Vitrolife）或 ISM（Medicult）培养基中。主要终点是受精率和胚胎形态率。
GIII 系列和 ISM 的受精率分别为（IVF：59.9% vs 62.0%和 ICSI：65.7% vs 66.8%），无差异。与 ISM 相比，GIII 系列显示早期分裂率增加，（IVF：25.8% vs 16.2%（p=0.005）；ICSI：40.8% vs 25.5%（p<0.0001），并且第 2 天的优质胚胎形态率更高[IVF：64.6% vs 57.3%（p=0.01）；ICSI：74.2% vs 69.4%（p=0.03）]和第 3 天[IVF：57.5% vs 49.0%（p=0.02）；ICSI：67.2% vs 61.6%（p=0.01）]。
当胚胎在 GIII 系列中培养时，第 2/3 天的胚胎形态显著增强。
