Sripreechasak Paranee, Tamura Tomohiko, Shibata Chiyo, Suwanborirux Khanit, Tanasupawat Somboon
Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University,Bangkok 10330,Thailand.
Biological Resource Centre, National Institute of Technology and Evaluation,2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0812,Japan.
Int J Syst Evol Microbiol. 2016 May;66(5):2030-2034. doi: 10.1099/ijsem.0.000987. Epub 2016 Feb 23.
A novel actinomycete, strain KC-112T, was isolated from soil collected from Similan Islands, Phang-Nga Province, Thailand. The strain exhibited morphological and chemotaxonomic characteristics consistent with those of members of the genus Streptomyces. The formation of smooth spiral spore chains was observed on aerial mycelia. ll-Diaminopimelic acid was detected in whole-cell hydrolysates, but no diagnostic sugars were detected and the strain lacked mycolic acids. The N-acyl type of muramic acid was acetyl. The major menaquinones were MK-9(H8), MK-9(H6) and MK-9(H2). The predominant cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0, iso-C16 : 0 and C16 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, an unknown phospholipid, an unknown aminolipid, unknown lipids and an unknown glycolipid. The DNA G+C content was 73 mol%. On the basis of 16S rRNA gene sequence analysis, strain KC-112T was closely related to Streptomyces fumanus NBRC 13042T (98.8 % 16S rRNA gene sequence similarity), Streptomyces anandii NBRC 13438T (98.8 %) and Streptomyces capillispiralis NBRC 14222T (98.8 %). DNA-DNA relatedness values among strain KC-112T and type strains of closely related species were lower than 70 %. On the basis of evidence from this taxonomic study using a polyphasic approach, strain KC-112T represents a novel species of the genus Streptomyces, namely Streptomyces andamanensis sp. nov. The type strain is KC-112T ( = KCTC 29502T = NBRC 110085T = PCU 347T = TISTR 2401T).
从泰国攀牙府斯米兰群岛采集的土壤中分离出一株新型放线菌，菌株KC-112T。该菌株表现出与链霉菌属成员一致的形态和化学分类特征。在气生菌丝体上观察到光滑螺旋状孢子链的形成。在全细胞水解物中检测到ll-二氨基庚二酸，但未检测到诊断性糖类，且该菌株缺乏分枝菌酸。胞壁酸的N-酰基类型为乙酰基。主要的甲基萘醌是MK-9(H8)、MK-9(H6)和MK-9(H2)。主要的细胞脂肪酸是anteiso-C15 : 0、anteiso-C17 : 0、iso-C16 : 0和C16 : 0。极性脂质为双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰肌醇、磷脂酰肌醇甘露糖苷、一种未知的磷脂、一种未知的氨基脂质、未知脂质和一种未知的糖脂。DNA G+C含量为73mol%。基于16S rRNA基因序列分析，菌株KC-112T与富马链霉菌NBRC 13042T（16S rRNA基因序列相似性为98.8%）、阿南德链霉菌NBRC 13438T（98.8%）和螺旋链丝菌NBRC 14222T（98.8%）密切相关。菌株KC-112T与密切相关物种的模式菌株之间的DNA-DNA相关性值低于70%。基于使用多相分类方法进行的这项分类学研究的证据，菌株KC-112T代表链霉菌属的一个新物种，即安达曼链霉菌新种。模式菌株为KC-112T（=KCTC 29502T =NBRC 110085T =PCU 347T =TISTR 2401T）。
