Sripreechasak Paranee, Phongsopitanun Wongsakorn, Tamura Tomohiko, Tanasupawat Somboon
Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
Int J Syst Evol Microbiol. 2017 Jan;67(1):50-54. doi: 10.1099/ijsem.0.001570. Epub 2017 Feb 20.
A novel actinomycete, designated strain KC-035T, was isolated from soil collected from Krung Ching Waterfall National Park, Nakhon Si Thammarat Province, Thailand. Its taxonomic position was determined using a polyphasic approach. The strain had morphological and chemotaxonomic properties typical of members of the genus Streptomyces: flexuous spore chain; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H6) and MK-9(H4) as menaquinones; diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside as phospholipids; anteiso-C15 : 0, C16 : 0, iso-C16 : 0, iso-C15 : 0 and iso-C14 : 0 as major cellular fatty acids; and DNA G+C content of 72 mol%. 16S rRNA gene sequence analysis revealed that strain KC-035T showed high similarity to Streptomyces albiflavescens n20T (99.16 %) and Streptomyces siamensis KC-038T (98.43 %) as well as formed a monophyletic clade with them in the phylogenetic tree. On the basis of comparison of phenotypic properties and the low level of DNA-DNA relatedness, strain KC-035T could be distinguished from its closely related Streptomyces species and is considered to represent a novel species of the genus Streptomyces, for which the name Streptomyces krungchingensis sp. nov. is proposed. The type strain is KC-035T (=NBRC 110087T=KCTC 29503T=TISTR 2402T).
从泰国那空是贪玛叻府空清瀑布国家公园采集的土壤中分离出一株新型放线菌，命名为菌株KC-035T。采用多相分类法确定其分类地位。该菌株具有链霉菌属成员典型的形态和化学分类学特征：孢子链弯曲；细胞壁肽聚糖中含有ll-二氨基庚二酸；以MK-9(H8)、MK-9(H6)和MK-9(H4)作为甲基萘醌；以二磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰肌醇和磷脂酰肌醇甘露糖苷作为磷脂；以anteiso-C15 : 0、C16 : 0、iso-C16 : 0、iso-C15 : 0和iso-C14 : 0作为主要细胞脂肪酸；DNA G+C含量为72 mol%。16S rRNA基因序列分析表明，菌株KC-035T与白色链霉菌n20T（99.16%）和暹罗链霉菌KC-038T（98.43%）具有高度相似性，并且在系统发育树中与它们形成一个单系分支。基于表型特征比较和低水平的DNA-DNA相关性，菌株KC-035T可与其密切相关链霉菌种区分开来，被认为代表链霉菌属的一个新物种，为此提出新名称链霉菌空清种（Streptomyces krungchingensis sp. nov.）。模式菌株为KC-035T（=NBRC 110087T=KCTC 29503T=TISTR 2402T）。
