用冠状病毒S蛋白假型化的慢病毒与猪冠状病毒细胞嗜性的比较。
Comparison of lentiviruses pseudotyped with S proteins from coronaviruses and cell tropisms of porcine coronaviruses.
作者信息
Wang Jingjing, Deng Feng, Ye Gang, Dong Wanyu, Zheng Anjun, He Qigai, Peng Guiqing
机构信息
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China.
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
出版信息
Virol Sin. 2016 Feb;31(1):49-56. doi: 10.1007/s12250-015-3690-4. Epub 2016 Feb 19.
Abstract
The surface glycoproteins of coronaviruses play an important role in receptor binding and cell entry. Different coronaviruses interact with their specific receptors to enter host cells. Lentiviruses pseudotyped with their spike proteins (S) were compared to analyze the entry efficiency of various coronaviruses. Our results indicated that S proteins from different coronaviruses displayed varied abilities to mediate pseudotyped virus infection. Furthermore, the cell tropisms of porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV) have been characterized by live and pseudotyped viruses. Both live and pseudoviruses could infected Vero- CCL-81 (monkey kidney), Huh-7 (human liver), and PK-15 (pig kidney) cells efficiently. CCL94 (cat kidney) cells could be infected efficiently by TGEV but not PEDV. Overall, our study provides new insights into the mechanisms of viral entry and forms a basis for antiviral drug screening.
摘要
冠状病毒的表面糖蛋白在受体结合和细胞进入过程中发挥着重要作用。不同的冠状病毒与它们特定的受体相互作用以进入宿主细胞。将携带其刺突蛋白（S）的慢病毒假型化，以比较各种冠状病毒的进入效率。我们的结果表明，来自不同冠状病毒的S蛋白在介导假型病毒感染方面表现出不同的能力。此外，猪流行性腹泻病毒（PEDV）和传染性胃肠炎病毒（TGEV）的细胞嗜性已通过活病毒和假型病毒进行了表征。活病毒和假病毒都能有效感染Vero-CCL-81（猴肾）、Huh-7（人肝）和PK-15（猪肾）细胞。CCL94（猫肾）细胞可被TGEV有效感染，但不能被PEDV感染。总体而言，我们的研究为病毒进入机制提供了新的见解，并为抗病毒药物筛选奠定了基础。
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