Tuin S A, Pourdeyhimi B, Loboa E G
Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, 4208 EB3, Campus Box 7115, Raleigh, NC 27695, USA.
Biomed Mater. 2016 Feb 23;11(1):015017. doi: 10.1088/1748-6041/11/1/015017.
Electrospun nonwovens have been used extensively for tissue engineering applications due to their inherent similarities with respect to fibre size and morphology to that of native extracellular matrix (ECM). However, fabrication of large scaffold constructs is time consuming, may require harsh organic solvents, and often results in mechanical properties inferior to the tissue being treated. In order to translate nonwoven based tissue engineering scaffold strategies to clinical use, a high throughput, repeatable, scalable, and economic manufacturing process is needed. We suggest that nonwoven industry standard high throughput manufacturing techniques (meltblowing, spunbond, and carding) can meet this need. In this study, meltblown, spunbond and carded poly(lactic acid) (PLA) nonwovens were evaluated as tissue engineering scaffolds using human adipose derived stem cells (hASC) and compared to electrospun nonwovens. Scaffolds were seeded with hASC and viability, proliferation, and differentiation were evaluated over the course of 3 weeks. We found that nonwovens manufactured via these industry standard, commercially relevant manufacturing techniques were capable of supporting hASC attachment, proliferation, and both adipogenic and osteogenic differentiation of hASC, making them promising candidates for commercialization and translation of nonwoven scaffold based tissue engineering strategies.
由于电纺非织造布在纤维尺寸和形态方面与天然细胞外基质（ECM）具有内在相似性，因此已广泛用于组织工程应用。然而，大型支架构建体的制造耗时，可能需要使用苛刻的有机溶剂，并且其机械性能往往低于所治疗的组织。为了将基于非织造布的组织工程支架策略转化为临床应用，需要一种高通量、可重复、可扩展且经济的制造工艺。我们认为非织造布行业标准的高通量制造技术（熔喷、纺粘和梳理）可以满足这一需求。在本研究中，使用人脂肪来源干细胞（hASC）对熔喷、纺粘和梳理的聚乳酸（PLA）非织造布作为组织工程支架进行了评估，并与电纺非织造布进行了比较。将hASC接种到支架上，并在3周内评估其活力、增殖和分化情况。我们发现，通过这些行业标准的、具有商业相关性的制造技术制造的非织造布能够支持hASC的附着、增殖以及hASC的成脂和成骨分化，使其成为基于非织造布支架的组织工程策略商业化和转化的有前景的候选材料。
