• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

利用II类内含子减弱归巢内切酶的体外和体内表达

Using Group II Introns for Attenuating the In Vitro and In Vivo Expression of a Homing Endonuclease.

作者信息

Guha Tuhin Kumar, Hausner Georg

机构信息

Department of Microbiology, University of Manitoba, Winnipeg, Canada.

出版信息

PLoS One. 2016 Feb 24;11(2):e0150097. doi: 10.1371/journal.pone.0150097. eCollection 2016.

DOI:10.1371/journal.pone.0150097
PMID:26909494
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4801052/
Abstract

In Chaetomium thermophilum (DSM 1495) within the mitochondrial DNA (mtDNA) small ribosomal subunit (rns) gene a group IIA1 intron interrupts an open reading frame (ORF) encoded within a group I intron (mS1247). This arrangement offers the opportunity to examine if the nested group II intron could be utilized as a regulatory element for the expression of the homing endonuclease (HEase). Constructs were generated where the codon-optimized ORF was interrupted with either the native group IIA1 intron or a group IIB type intron. This study showed that the expression of the HEase (in vivo) in Escherichia coli can be regulated by manipulating the splicing efficiency of the HEase ORF-embedded group II introns. Exogenous magnesium chloride (MgCl2) stimulated the expression of a functional HEase but the addition of cobalt chloride (CoCl2) to growth media antagonized the expression of HEase activity. Ultimately the ability to attenuate HEase activity might be useful in precision genome engineering, minimizing off target activities, or where pathways have to be altered during a specific growth phase.

摘要

在嗜热毛壳菌（DSM 1495）的线粒体DNA（mtDNA）小核糖体亚基（rns）基因中，一个IIA1组内含子打断了一个编码在I组内含子（mS1247）中的开放阅读框（ORF）。这种排列提供了一个机会来研究嵌套的II组内含子是否可以用作归巢内切酶（HEase）表达的调控元件。构建了这样的载体，其中密码子优化的ORF被天然的IIA1组内含子或IIB型内含子打断。这项研究表明，通过操纵嵌入HEase ORF的II组内含子的剪接效率，可以在体内调控大肠杆菌中HEase的表达。外源性氯化镁（MgCl2）刺激了功能性HEase的表达，但向生长培养基中添加氯化钴（CoCl2）会拮抗HEase活性的表达。最终，减弱HEase活性的能力可能在精确基因组工程、最小化脱靶活性或在特定生长阶段必须改变途径的情况下有用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aa5/4801052/c1eae1835364/pone.0150097.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aa5/4801052/7fd4806e923f/pone.0150097.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aa5/4801052/9b5d0bd0c830/pone.0150097.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aa5/4801052/627fa6d98b35/pone.0150097.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aa5/4801052/6013336ddcb8/pone.0150097.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aa5/4801052/05935d8cef83/pone.0150097.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aa5/4801052/c1eae1835364/pone.0150097.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aa5/4801052/7fd4806e923f/pone.0150097.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aa5/4801052/9b5d0bd0c830/pone.0150097.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aa5/4801052/627fa6d98b35/pone.0150097.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aa5/4801052/6013336ddcb8/pone.0150097.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aa5/4801052/05935d8cef83/pone.0150097.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aa5/4801052/c1eae1835364/pone.0150097.g006.jpg

相似文献

1
Using Group II Introns for Attenuating the In Vitro and In Vivo Expression of a Homing Endonuclease.利用II类内含子减弱归巢内切酶的体外和体内表达
PLoS One. 2016 Feb 24;11(2):e0150097. doi: 10.1371/journal.pone.0150097. eCollection 2016.
2
Insertion of Group II Intron-Based Ribozyme Switches into Homing Endonuclease Genes.将基于II组内含子的核酶开关插入归巢内切酶基因。
Methods Mol Biol. 2017;1498:135-152. doi: 10.1007/978-1-4939-6472-7_9.
3
A homing endonuclease with a switch: characterization of a twintron encoded homing endonuclease.具有开关的归巢内切酶：双内含子编码归巢内切酶的表征。
Fungal Genet Biol. 2014 Apr;65:57-68. doi: 10.1016/j.fgb.2014.01.004. Epub 2014 Feb 5.
4
The mtDNA rns gene landscape in the Ophiostomatales and other fungal taxa: twintrons, introns, and intron-encoded proteins.Ophiostomatales 及其他真菌分类单元中的 mtDNA rns 基因景观：双内含子、内含子和内含子编码蛋白。
Fungal Genet Biol. 2013 Apr;53:71-83. doi: 10.1016/j.fgb.2013.01.005. Epub 2013 Feb 9.
5
Characterization of the I-Spom I endonuclease from fission yeast: insights into the evolution of a group I intron-encoded homing endonuclease.裂殖酵母中I-Spom I内切核酸酶的特性：对I类内含子编码归巢内切核酸酶进化的见解
J Mol Evol. 2002 Sep;55(3):302-13. doi: 10.1007/s00239-001-2327-4.
6
I-OmiI and I-OmiII: two intron-encoded homing endonucleases within the Ophiostoma minus rns gene.I-OmiI和I-OmiII：微小长喙壳菌rns基因内的两个内含子编码归巢内切酶。
Fungal Biol. 2014 Aug;118(8):721-31. doi: 10.1016/j.funbio.2014.05.002. Epub 2014 May 23.
7
I-ApeKI [corrected]: a novel intron-encoded LAGLIDADG homing endonuclease from the archaeon, Aeropyrum pernix K1.I-ApeKI[已修正]：一种来自古菌嗜热栖热菌K1的新型内含子编码LAGLIDADG归巢内切酶。
Nucleic Acids Res. 2005 Jul 26;33(13):e116. doi: 10.1093/nar/gni118.
8
The diversity of mtDNA rns introns among strains of Ophiostoma piliferum, Ophiostoma pluriannulatum and related species.松材线虫病菌、多环拟层孔菌及相关物种菌株中线粒体DNA核糖体RNA内含子的多样性。
Springerplus. 2016 Aug 24;5(1):1408. doi: 10.1186/s40064-016-3076-6. eCollection 2016.
9
The intron landscape of the mtDNA cytb gene among the Ascomycota: introns and intron-encoded open reading frames.子囊菌门线粒体细胞色素b基因的内含子图谱：内含子和内含子编码的开放阅读框
Mitochondrial DNA A DNA Mapp Seq Anal. 2018 Oct;29(7):1015-1024. doi: 10.1080/24701394.2017.1404042. Epub 2017 Nov 20.
10
[Cloning and expressing of cellulase gene (cbh2) from thermophilic fungi Chaetomium thermophilum CT2].嗜热毛壳菌CT2纤维素酶基因（cbh2）的克隆与表达
Sheng Wu Gong Cheng Xue Bao. 2005 Nov;21(6):892-9.

引用本文的文献

1
Organellar Introns in Fungi, Algae, and Plants.细胞器基因在真菌、藻类和植物中的分布。
Cells. 2021 Aug 6;10(8):2001. doi: 10.3390/cells10082001.
2
The Mitogenomes of and and Comparisons With Other Members of the Ophiostomatales.[物种名称1]和[物种名称2]的线粒体基因组及其与长喙壳目其他成员的比较。
Front Microbiol. 2021 Feb 10;12:618649. doi: 10.3389/fmicb.2021.618649. eCollection 2021.
3
Programmable Genome Editing Tools and their Regulation for Efficient Genome Engineering.用于高效基因组工程的可编程基因组编辑工具及其调控

本文引用的文献

1
Use of transposase and ends of IS608 enables precise and scarless genome modification for modulating gene expression and metabolic engineering applications in Escherichia coli.转座酶和IS608末端的使用能够实现精确且无疤痕的基因组修饰，用于调节大肠杆菌中的基因表达和代谢工程应用。
Biotechnol J. 2016 Jan;11(1):80-90. doi: 10.1002/biot.201500205. Epub 2015 Sep 18.
2
Retrohoming of a Mobile Group II Intron in Human Cells Suggests How Eukaryotes Limit Group II Intron Proliferation.人细胞中一个可移动的II类内含子的反向归巢揭示了真核生物限制II类内含子增殖的方式。
PLoS Genet. 2015 Aug 4;11(8):e1005422. doi: 10.1371/journal.pgen.1005422. eCollection 2015 Aug.
3
Comput Struct Biotechnol J. 2017 Jan 12;15:146-160. doi: 10.1016/j.csbj.2016.12.006. eCollection 2017.
4
The diversity of mtDNA rns introns among strains of Ophiostoma piliferum, Ophiostoma pluriannulatum and related species.松材线虫病菌、多环拟层孔菌及相关物种菌株中线粒体DNA核糖体RNA内含子的多样性。
Springerplus. 2016 Aug 24;5(1):1408. doi: 10.1186/s40064-016-3076-6. eCollection 2016.
Metabolic engineering of Escherichia coli using CRISPR-Cas9 meditated genome editing.
利用CRISPR-Cas9介导的基因组编辑对大肠杆菌进行代谢工程改造。
Metab Eng. 2015 Sep;31:13-21. doi: 10.1016/j.ymben.2015.06.006. Epub 2015 Jun 30.
4
Mobile Bacterial Group II Introns at the Crux of Eukaryotic Evolution.移动细菌组 II 内含子是真核生物进化的关键。
Microbiol Spectr. 2015 Feb;3(1):MDNA3-0050-2014. doi: 10.1128/microbiolspec.MDNA3-0050-2014.
5
Photoactivatable CRISPR-Cas9 for optogenetic genome editing.光激活 CRISPR-Cas9 用于光遗传学基因组编辑。
Nat Biotechnol. 2015 Jul;33(7):755-60. doi: 10.1038/nbt.3245. Epub 2015 Jun 15.
6
Expanding the Biologist's Toolkit with CRISPR-Cas9.利用 CRISPR-Cas9 扩展生物学家的工具包。
Mol Cell. 2015 May 21;58(4):568-74. doi: 10.1016/j.molcel.2015.02.032.
7
Evolution of group II introns.II类内含子的进化。
Mob DNA. 2015 Apr 1;6:7. doi: 10.1186/s13100-015-0037-5. eCollection 2015.
8
The Phyre2 web portal for protein modeling, prediction and analysis.用于蛋白质建模、预测和分析的Phyre2网络门户。
Nat Protoc. 2015 Jun;10(6):845-58. doi: 10.1038/nprot.2015.053. Epub 2015 May 7.
9
Small molecule-triggered Cas9 protein with improved genome-editing specificity.小分子触发的 Cas9 蛋白，提高了基因组编辑的特异性。
Nat Chem Biol. 2015 May;11(5):316-8. doi: 10.1038/nchembio.1793. Epub 2015 Apr 6.
10
Magnesium uptake of Arabidopsis transporters, AtMRS2-10 and AtMRS2-11, expressed in Escherichia coli mutants: Complementation and growth inhibition by aluminum.在大肠杆菌突变体中表达的拟南芥转运蛋白AtMRS2 - 10和AtMRS2 - 11对镁的摄取：铝的互补作用和生长抑制
Biochim Biophys Acta. 2015 Jun;1848(6):1376-82. doi: 10.1016/j.bbamem.2015.03.005. Epub 2015 Mar 13.