Soares Maria, Ribeiro Rita, Najmudin Shabir, Gameiro Andreia, Rodrigues Rita, Cardoso Fátima, Ferreira Fernando
CIISA, Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal.
Breast Unit, Champalimaud Clinical Center, Lisbon, Portugal.
Oncotarget. 2016 Apr 5;7(14):17314-26. doi: 10.18632/oncotarget.7551.
HER2 is overexpressed in about 30% of feline mammary carcinomas (FMC) and in 15-30% of breast cancers. Women with HER2-positive breast tumors are associated with shorter survival. This study aimed to optimize the detection and quantification of serum HER2 (sHER2) in cats and to evaluate its potential in diagnosing cats with mammary carcinomas (MC) overexpressing HER2. A prospective study was conducted in 60 queens showing MC and 20 healthy animals. Pre-operative serum samples were collected for sHER2 quantification using two immunoassays: ELISA and Dot blot assay. sHER2 levels were compared with tissue HER2 status assessed by immunohistochemistry. Queens with FMC showed significantly higher mean levels of sHER2 by both ELISA and Dot blot assay. A significant difference in the sHER2 levels was also found between cats with HER2-positive MC and those with low-expressing HER2 MC. A significant correlation between sHER2 levels and tumor HER2 status was also found, particularly when ELISA was used (r = 0.58, p < 0.0001). The value of 10 ng/ml was proposed as the optimal cutoff for both immunoassays by ROC analysis. Like in humans, sHER2 levels are increased in cats with MC HER2-positive, strongly suggesting that evaluation of sHER2 levels can be very useful in feline oncology. The results show that ELISA and Dot blot assay can replace the immunohistochemistry technique, due to their efficacy and lower costs for diagnostic purposes and for monitoring the response to anti-HER2 therapies in cats.
HER2在约30%的猫乳腺肿瘤（FMC）和15 - 30%的乳腺癌中过表达。HER2阳性乳腺肿瘤的女性患者生存期较短。本研究旨在优化猫血清HER2（sHER2）的检测和定量，并评估其在诊断HER2过表达的猫乳腺癌（MC）中的潜力。对60只患有MC的母猫和20只健康动物进行了一项前瞻性研究。术前采集血清样本，使用两种免疫测定法进行sHER2定量：酶联免疫吸附测定（ELISA）和斑点印迹法。将sHER2水平与通过免疫组织化学评估的组织HER2状态进行比较。患有FMC的母猫通过ELISA和斑点印迹法检测的sHER2平均水平均显著更高。在HER2阳性MC的猫和HER2低表达MC的猫之间，sHER2水平也存在显著差异。还发现sHER2水平与肿瘤HER2状态之间存在显著相关性，特别是在使用ELISA时（r = 0.58，p < 0.0001）。通过ROC分析，建议将10 ng/ml的值作为两种免疫测定法的最佳临界值。与人类一样，HER2阳性MC的猫的sHER2水平升高，强烈表明评估sHER2水平在猫肿瘤学中可能非常有用。结果表明，ELISA和斑点印迹法可替代免疫组织化学技术，因为它们在诊断目的以及监测猫抗HER2治疗反应方面具有有效性且成本较低。
