Wang Chuanxi, Lin Huihui, Xu Zhenzhu, Huang Yijun, Humphrey Mark G, Zhang Chi
China-Australia Joint Research Centre for Functional Molecular Materials, School of Chemical & Material Engineering, Jiangnan University , Wuxi 214122, People's Republic of China.
Research School of Chemistry, Australian National University , Canberra, ACT 2601, Australia.
ACS Appl Mater Interfaces. 2016 Mar;8(10):6621-8. doi: 10.1021/acsami.5b11317. Epub 2016 Mar 2.
The use of carbon-dot-based dual-emission fluorescent nanohybrids (DEFNs) as versatile nanothermometry devices for spatially resolved temperature measurements in living cells is demonstrated. The carbon dots (CDs) are prepared in the organic phase and display tunable photoluminescence (PL) across a wide visible range by adjusting the excitation wavelengths and extend of N-doping. DEFNs are formed in a straightforward fashion from CDs (emitting blue PL) and gold nanoclusters (AuNCs, emitting red PL). The DEFNs display ideal single-excitation, dual-emission with two well-resolved, intensity-comparable fluorescence peaks, and function in optical thermometry with high reliability and accuracy by exploiting the temperature sensitivity of their fluorescence intensity ratio (blue/red). Furthermore, the DEFNs have been introduced into cells, exhibiting good biocompatibility, and have facilitated physiological temperature measurements in the range of 25-45 °C; the DEFNs can therefore function as "non-contact" tools for the accurate measurement of temperature and its gradient inside a living cell.
本文展示了基于碳点的双发射荧光纳米杂化物（DEFNs）作为多功能纳米温度计用于活细胞中空间分辨温度测量的应用。碳点（CDs）在有机相中制备，并通过调节激发波长和氮掺杂程度在宽可见范围内显示出可调谐的光致发光（PL）。DEFNs由发射蓝色PL的CDs和发射红色PL的金纳米簇（AuNCs）以直接的方式形成。DEFNs表现出理想的单激发、双发射，具有两个分辨率良好、强度可比的荧光峰，并通过利用其荧光强度比（蓝色/红色）的温度敏感性在光学测温中具有高可靠性和准确性。此外，DEFNs已被引入细胞中，表现出良好的生物相容性，并有助于在25 - 45°C范围内进行生理温度测量；因此，DEFNs可以作为“非接触”工具，用于精确测量活细胞内的温度及其梯度。
