Wang Xin, Luo Yan, Masci Paul P, Crawford Ross, Xiao Yin
Department of Spine, Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou Province, China.
Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia.
PLoS One. 2016 Feb 24;11(2):e0149775. doi: 10.1371/journal.pone.0149775. eCollection 2016.
Hematoma quality (especially the fibrin matrix) plays an important role in the bone healing process. Here, we investigated the effect of interleukin-1 beta (IL-1β) on fibrin clot formation from platelet-poor plasma (PPP).
Five-milliliter of rat whole-blood samples were collected from the hepatic portal vein. All blood samples were firstly standardized via a thrombelastograph (TEG), blood cell count, and the measurement of fibrinogen concentration. PPP was prepared by collecting the top two-fifths of the plasma after centrifugation under 400 × g for 10 min at 20°C. The effects of IL-1β cytokines on artificial fibrin clot formation from PPP solutions were determined by scanning electronic microscopy (SEM), confocal microscopy (CM), turbidity, and clot lysis assays.
The lag time for protofibril formation was markedly shortened in the IL-1β treatment groups (243.8 ± 76.85 in the 50 pg/mL of IL-1β and 97.5 ± 19.36 in the 500 pg/mL of IL-1β) compared to the control group without IL-1β (543.8 ± 205.8). Maximal turbidity was observed in the control group. IL-1β (500 pg/mL) treatment significantly decreased fiber diameters resulting in smaller pore sizes and increased density of the fibrin clot structure formed from PPP (P < 0.05). The clot lysis assay revealed that 500 pg/mL IL-1β induced a lower susceptibility to dissolution due to the formation of thinner and denser fibers.
IL-1β can significantly influence PPP fibrin clot structure, which may affect the early bone healing process.
血肿质量（尤其是纤维蛋白基质）在骨愈合过程中起重要作用。在此，我们研究了白细胞介素 -1β（IL-1β）对乏血小板血浆（PPP）纤维蛋白凝块形成的影响。
从大鼠肝门静脉采集5毫升全血样本。所有血样首先通过血栓弹力图（TEG）、血细胞计数和纤维蛋白原浓度测量进行标准化。通过在20°C下以400×g离心10分钟后收集上层五分之二的血浆来制备PPP。通过扫描电子显微镜（SEM）、共聚焦显微镜（CM）、比浊法和凝块溶解试验确定IL-1β细胞因子对PPP溶液人工纤维蛋白凝块形成的影响。
与无IL-1β的对照组（543.8±205.8）相比，IL-1β治疗组原纤维形成的延迟时间明显缩短（50 pg/mL IL-1β组为243.8±76.85，500 pg/mL IL-1β组为97.5±19.36）。对照组观察到最大比浊度。IL-1β（500 pg/mL）处理显著降低了纤维直径，导致孔径变小，由PPP形成的纤维蛋白凝块结构密度增加（P<0.05）。凝块溶解试验表明，500 pg/mL IL-1β由于形成更细且更致密的纤维而导致溶解敏感性降低。
IL-1β可显著影响PPP纤维蛋白凝块结构，这可能会影响早期骨愈合过程。
