Foundation Eduardo Anitua, Vitoria, Spain.
BTI-Biotechnology Institute, Vitoria, Spain.
J Mater Sci Mater Med. 2017 Dec 14;29(1):7. doi: 10.1007/s10856-017-6012-6.
Biomaterials should be designed to closely resemble the characteristics and functions of the native extracellular matrix to provide mechanical support and signals to direct biological events. Here we have developed a novel injectable plasma rich in growth factors (PRGF-Endoret)-based formulation that combines a thermal-denaturation step of plasma with an autologous fibrin crosslinking. Rheological and mechanical properties were evaluated. Additionally, the microstructure and biological capacity of the biomaterial was also characterized. This novel formulation exhibited ideal mechanical properties and a gel-like behavior with the ability to progressively release its growth factor load over time. The results also suggested that the novel injectable formulation is non-cytotoxic, biocompatible and suitable for cell ingrowth as it is deduced from the fibroblast proliferation within the scaffold. Finally, stimulation of both cell proliferation and matrix proteins synthesis demonstrated the regenerative potential of this autologous protein based injectable scaffold.
生物材料的设计应尽可能模仿天然细胞外基质的特性和功能，为生物事件提供机械支撑和信号。在这里，我们开发了一种新型的富含生长因子的可注射血浆（PRGF-Endoret）制剂，该制剂将等离子体的热变性步骤与自体纤维蛋白交联相结合。评估了流变学和力学性能。此外，还对生物材料的微观结构和生物学性能进行了表征。这种新型制剂表现出理想的机械性能和凝胶样行为，随着时间的推移能够逐步释放其生长因子负载。结果还表明，新型可注射制剂无细胞毒性、生物相容性，适合细胞向内生长，因为可以从支架内的成纤维细胞增殖推断出来。最后，细胞增殖和基质蛋白合成的刺激表明这种基于自体蛋白的可注射支架具有再生潜力。
