Li M, Zhao S Z, Zhao C Z, Zhang Y, Xia H, Lopez-Baltazar J, Wan S B, Wang X J
Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan, Shandong Province, China.
Instituto Tecnológico del Valle de Oaxaca, Oaxaca, Mexico.
Genet Mol Res. 2016 Feb 19;15(1):gmr7344. doi: 10.4238/gmr.15017344.
SQUAMOSA promoter-binding protein-like (SPL) proteins play crucial roles in plant growth, development, and responses to environmental stressors. The peanut (Arachis hypogaea L.) is a globally important oil crop. In this study, we cloned the full-length cDNA of 15 SPLs in the peanut by transcriptome sequencing and rapid amplification of cDNA ends, and analyzed their genomic DNA sequences. cDNA lengths varied significantly, from 369 to 3102 bp. The SBP domain of the peanut SPL proteins was highly conserved compared to SPLs in other plant species. Based on their sequence similarity to SPLs from other plant species, the peanut SPLs could be grouped into five subgroups. In each subgroup, lengths of individual genes, conserved motif numbers, and distribution patterns were similar. Seven of the SPLs were predicted to be targets of miR156. The SPLs were ubiquitously expressed in the roots, leaves, flowers, gynophores, and seeds, with different expression levels and accumulation patterns. Significant differences in the expression of most of the SPLs were observed between juvenile and adult leaves, suggesting that they are involved in developmental regulation. Dynamic changes occurred in transcript levels at stage 1 (aerial grown green gynophores), stage 2 (gynophores buried in soil for about three days), and stage 3 (gynophores buried in soil for about nine days with enlarged pods). Possible roles that these genes play in peanut pod initiation are discussed.
SQUAMOSA启动子结合蛋白样（SPL）蛋白在植物生长、发育以及对环境胁迫的响应中发挥着关键作用。花生（Arachis hypogaea L.）是一种全球重要的油料作物。在本研究中，我们通过转录组测序和cDNA末端快速扩增技术克隆了花生中15个SPL的全长cDNA，并分析了它们的基因组DNA序列。cDNA长度差异显著，从369 bp到3102 bp不等。与其他植物物种中的SPL相比，花生SPL蛋白的SBP结构域高度保守。基于它们与其他植物物种SPL的序列相似性，花生SPL可分为五个亚组。在每个亚组中，各个基因的长度、保守基序数量和分布模式相似。其中7个SPL被预测为miR156的靶标。这些SPL在根、叶、花、果针和种子中均有表达，且表达水平和积累模式各不相同。在幼叶和成熟叶之间观察到大多数SPL的表达存在显著差异，表明它们参与发育调控。在第1阶段（地上生长的绿色果针）、第2阶段（果针埋入土壤约三天）和第3阶段（果针埋入土壤约九天且荚果膨大），转录水平发生了动态变化。本文还讨论了这些基因在花生荚果起始过程中可能发挥的作用。
