Mouri Israt Jahan, Islam Md Shariful
Faculty of Biotechnology and Genetic Engineering, Sylhet Agricultural University, Sylhet 3100, Bangladesh.
Faculty of Biotechnology and Genetic Engineering, Sylhet Agricultural University, Sylhet 3100, Bangladesh; Department of Molecular Biology and Genetic Engineering, Sylhet Agricultural University, Sylhet 3100, Bangladesh.
J Genet Eng Biotechnol. 2025 Mar;23(1):100461. doi: 10.1016/j.jgeb.2025.100461. Epub 2025 Jan 23.
One of the largest and most significant transcription factor gene families in plants is the SQUAMOSA promoter binding protein (SBP) gene family and they perform critical regulatory roles in floral enhancement, fruit development, and stress resistance. The SBP protein family (also known as SPL) has not yet been thoroughly studied in the staple fruit crop, banana. A perennial monocot plant, banana is essential for ensuring food and nutrition security. This work detected 41 SBP genes in the banana species Musa acuminata. The MaSBPs were subsequently elucidated by investigating their gene structure, chromosomal position, RNA-Seq data, along with evolutionary connections with Arabidopsis and rice. Sequence alignment of MaSBPs revealed that all genes included a domain of two Zn finger motifs (CCCH and CCHC motifs) with an overlapping nuclear localization signal region. The conserved motifs sequence in the inferred MaSBP proteins were quite comparable. According to findings, the time frame of divergence for duplicated MaSBP gene pairs ranged from 42.39 to 109.11 million years and the dicot Arabidopsis and monocotyledonous plant banana diverged before the division of banana and monocot rice. Moreover, cis-acting element and GO annotation analysis exhibited possible biological activities of MaSBPs in flower development, phytohormone regulation, and stress tolerance. RNA-Seq expression profiling exhibited that genes MaSBP-3, MaSBP-20, MaSBP-37, MaSBP-40 were more expressed during floral and fruit development stage. The foundation for additional investigation of SBP protein sequences in other plants can be laid out by this study, which will shed light on some of their crucial biological functions.
植物中最大且最重要的转录因子基因家族之一是SQUAMOSA启动子结合蛋白（SBP）基因家族，它们在花发育、果实发育和抗逆性中发挥着关键的调控作用。SBP蛋白家族（也称为SPL）在主要水果作物香蕉中尚未得到充分研究。香蕉作为一种多年生单子叶植物，对于确保粮食和营养安全至关重要。这项研究在香蕉品种小果野蕉中检测到41个SBP基因。随后，通过研究它们的基因结构、染色体位置、RNA测序数据以及与拟南芥和水稻的进化关系，对MaSBP进行了阐明。MaSBP的序列比对显示，所有基因都包含一个由两个锌指基序（CCCH和CCHC基序）组成的结构域以及一个重叠的核定位信号区域。推断的MaSBP蛋白中的保守基序序列相当相似。研究结果表明，重复的MaSBP基因对的分歧时间范围为4239万年至1.0911亿年，双子叶植物拟南芥和单子叶植物香蕉在香蕉和单子叶植物水稻分化之前就已经分化。此外，顺式作用元件和基因本体注释分析显示了MaSBP在花发育、植物激素调节和胁迫耐受性方面可能的生物学活性。RNA测序表达谱分析表明，基因MaSBP - 3、MaSBP - 20、MaSBP - 37、MaSBP - 40在花和果实发育阶段表达量更高。这项研究可以为进一步研究其他植物中的SBP蛋白序列奠定基础，这将有助于揭示它们的一些关键生物学功能。
