Center for Research and Development of Fine Chemicals, Guizhou University, Guiyang, 550025, China; Institute of Medicinal Plant Development, the Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100193, China; Qiannan Medical College for Nationalities, Duyun, 558003, China.
J Integr Plant Biol. 2014 Jan;56(1):38-50. doi: 10.1111/jipb.12111. Epub 2013 Nov 20.
SQUAMOSA promoter binding protein-likes (SPLs) are plant-specific transcription factors playing vital regulatory roles in plant growth and development. There is no information about SPLs in Salvia miltiorrhiza (Danshen), a significant medicinal plant widely used in Traditional Chinese medicine (TCM) for >1,700 years and an emerging model plant for TCM studies. Through genome-wide identification and subsequent molecular cloning, we identified a total 15 SmSPLs with divergent sequence features, gene structures, and motifs. Comparative analysis showed sequence conservation between SmSPLs and their Arabidopsis counterparts. A phylogenetic tree clusters SmSPLs into six groups. Many of the motifs identified commonly exist in a group/subgroup, implying their functional redundancy. Eight SmSPLs were predicted and experimentally validated to be targets of miR156/157. SmSPLs were differentially expressed in various tissues of S. milltiorrhiza. The expression of miR156/157-targeted SmSPLs was increased with the maturation of S. miltiorrhiza, whereas the expression of miR156/157 was decreased, confirming the regulatory roles of miR156/157 in SmSPLs and suggesting the functions of SmSPLs in S. miltiorrhiza development. The expression of miR156/157 was negatively correlated with miR172 during the maturation of S. miltiorrhiza. The results indicate the significance and complexity of SmSPL-, miR156-, and miR172-mediated regulation of developmental timing in S. miltiorrhiza.
SQUAMOSA 启动子结合蛋白类似物（SPLs）是植物特有的转录因子，在植物生长和发育中发挥着重要的调节作用。丹参（Danshen）是一种重要的药用植物，在中国传统医学（TCM）中已有 1700 多年的应用历史，也是 TCM 研究的新兴模式植物，但目前尚无关于丹参 SPLs 的信息。通过全基因组鉴定和随后的分子克隆，我们共鉴定出 15 个具有不同序列特征、基因结构和基序的 SmSPLs。比较分析显示 SmSPLs 与拟南芥同源物之间存在序列保守性。系统发育树将 SmSPLs 聚类为六个组。许多鉴定出的基序普遍存在于一个组/亚组中，这暗示了它们的功能冗余性。预测并实验验证了 8 个 SmSPLs 是 miR156/157 的靶标。SmSPLs 在丹参的各种组织中差异表达。随着丹参的成熟，miR156/157 靶向的 SmSPLs 的表达增加，而 miR156/157 的表达减少，证实了 miR156/157 在 SmSPLs 中的调节作用，并提示了 SmSPLs 在丹参发育中的功能。miR156/157 在丹参成熟过程中的表达与 miR172 呈负相关。这些结果表明 SmSPL、miR156 和 miR172 介导的调控在丹参发育中的时间调控中的重要性和复杂性。
