经皮给予大豆乙基吗啉乙磺酸酯胶束对葡萄球菌属的抗菌活性。
Antimicrobial activity of topically-applied soyaethyl morpholinium ethosulfate micelles against Staphylococcus species.
机构信息
Pharmaceutics Laboratory, Graduate Institute of Natural Products, Chang Gung University, 259 Wen-Hwa 1st Road, Kweishan, Taoyuan 333, Taiwan.
Research Center for Industry of Human Ecology, Chang Gung University of Science & Technology, Kweishan, Taoyuan, Taiwan.
出版信息
Nanomedicine (Lond). 2016 Mar;11(6):657-71. doi: 10.2217/nnm.15.217. Epub 2016 Feb 25.
AIM
Here we evaluated the antibacterial efficacy of soyaethyl morpholinium ethosulfate (SME) micelles as an inherent bactericide against Staphylococcus aureus and methicillin-resistant S. aureus (MRSA).
METHODOLOGY
The antimicrobial activity was examined by in vitro culture model and murine model of skin infection. Cationic micelles formed by benzalkonium chloride or cetylpyridinium chloride were used for comparison.
RESULTS
The minimum inhibitory concentration and minimum bactericidal concentration against S. aureus and MRSA were 1.71-3.42 and 1.71-6.84 μg/ml, respectively. Topical administration of SME micelles significantly decreased the cutaneous infection and MRSA load in mice. The killing of bacteria was caused by direct cell wall/membrane rupture. SME micelles also penetrated into the bacteria to elicit a Fenton reaction and oxidative stress.
CONCLUSION
SME micelles have potential as antimicrobial agents due to their lethal effect against S. aureus and MRSA with a low toxicity to mammalian cells.
目的
本研究旨在评估大豆乙基吗啉乙磺酸盐（SME）胶束作为固有杀菌剂对金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌（MRSA）的抗菌功效。
方法
通过体外培养模型和皮肤感染的小鼠模型来检测抗菌活性。并使用苯扎氯铵或十六烷基吡啶氯形成的阳离子胶束进行比较。
结果
SME 胶束对金黄色葡萄球菌和 MRSA 的最低抑菌浓度和最低杀菌浓度分别为 1.71-3.42 和 1.71-6.84 μg/ml。局部给予 SME 胶束可显著降低小鼠皮肤感染和 MRSA 负荷。细菌的杀灭是由直接的细胞壁/膜破裂引起的。SME 胶束还能进入细菌引发芬顿反应和氧化应激。
结论
由于 SME 胶束对金黄色葡萄球菌和 MRSA 具有致死作用，且对哺乳动物细胞的毒性较低，因此具有作为抗菌剂的潜力。
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