大肠杆菌素Ia的作用模式：大肠杆菌素对大肠杆菌质子电化学梯度的影响。
Mode of action of colicin Ia: effect of colicin on the Escherichia coli proton electrochemical gradient.
作者信息
Tokuda H, Konisky J
出版信息
Proc Natl Acad Sci U S A. 1978 Jun;75(6):2579-83. doi: 10.1073/pnas.75.6.2579.
Abstract
By use of the technique of flow dialysis, the membrane potential (deltapsi) and pH gradient (deltapH) have been measured in colicin Ia-treated Escherichia coli K-12 cells and in membrane vesicles prepared from such cells. Although such cells and vesicles are able to generate a transmembrane deltapH at pH 5.5, they do not generate a transmembrane deltapsi. Glucose-6-phospate uptake by cells is shown to be stimulated at pH 5.5 and inhibited at pH 7.5 by colicin Ia treatment. On the other hand, proline uptake is demonstrated to be inhibited progressively at pH 5.5, 6.6, and 7.5 in colicin Ia-treated cells. These data provide strong evidence for a colicin Ia-induced membrane deplorization and indicate that the membrane becomes permeable to ion(s) other than protons after treatment with colicin Ia.
摘要
通过使用流动透析技术，已在经大肠菌素Ia处理的大肠杆菌K - 12细胞以及由此类细胞制备的膜囊泡中测量了膜电位（Δψ）和pH梯度（ΔpH）。尽管此类细胞和囊泡能够在pH 5.5时产生跨膜ΔpH，但它们不会产生跨膜Δψ。结果表明，经大肠菌素Ia处理后，细胞对葡萄糖 - 6 - 磷酸的摄取在pH 5.5时受到刺激，而在pH 7.5时受到抑制。另一方面，在经大肠菌素Ia处理的细胞中，脯氨酸摄取在pH 5.5、6.6和7.5时均被证明逐渐受到抑制。这些数据为大肠菌素Ia诱导的膜去极化提供了有力证据，并表明在用大肠菌素Ia处理后，膜对除质子以外的离子变得具有通透性。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Calculation of intracellular pH from the distribution of 5,5-dimethyl-2,4-oxazolidinedione (DMO); application to skeletal muscle of the dog.根据5,5-二甲基-2,4-恶唑烷二酮（DMO）的分布计算细胞内pH值；应用于犬的骨骼肌
J Clin Invest. 1959 May;38(5):720-9. doi: 10.1172/JCI103852.
3
The role of energy coupling in the transport of beta-galactosides by Escherichia coli.能量偶联在大肠杆菌转运β-半乳糖苷中的作用。
J Biol Chem. 1966 May 25;241(10):2200-11.
4
Chemiosmotic coupling in oxidative and photosynthetic phosphorylation.氧化磷酸化和光合磷酸化中的化学渗透偶联
Biol Rev Camb Philos Soc. 1966 Aug;41(3):445-502. doi: 10.1111/j.1469-185x.1966.tb01501.x.
5
Mechanisms of active transport in isolated membrane vesicles. II. The mechanism of energy coupling between D-lactic dehydrogenase and beta-galactoside transport in membrane preparations from Escherichia coli.分离的膜泡中主动运输的机制。II. 大肠杆菌膜制剂中D-乳酸脱氢酶与β-半乳糖苷运输之间的能量偶联机制。
J Biol Chem. 1971 Sep 10;246(17):5523-31.
6
Characterization of colicin Ia and colicin Ib. Purification and some physical properties.大肠菌素Ia和大肠菌素Ib的特性。纯化及一些物理性质。
J Biol Chem. 1970 Jun 10;245(11):2972-8.
7
Transport in isolated bacterial membrane vesicles.在分离的细菌膜囊泡中的运输。
Methods Enzymol. 1974;31:698-709. doi: 10.1016/0076-6879(74)31075-0.
8
9
Performance and conservation of osmotic work by proton-coupled solute porter systems.质子偶联溶质转运体系统的渗透功的表现与守恒
J Bioenerg. 1973 Jan;4(1):63-91. doi: 10.1007/BF01516051.
10
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J Biol Chem. 1977 Aug 10;252(15):5491-7.
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